摘要
[目的]研究金水乌鸡PRL基因多态性,为金水乌鸡的保种和持续选育与提高积累分子基础数据。[方法]采用PCR-RFLP方法研究金水乌鸡白羽丝毛系165只乌鸡PRL基因多态性,分别采用HaeⅢ和HhaⅠ2种内切酶对各DNA样品的PCR产物进行单酶切,分析基因型与产蛋数的相关。[结果]金水乌鸡PRL基因的PCR产物的HhaⅠ酶切片段未出现多态性。金水乌鸡PRL基因的PCR产物的HaeⅢ酶切片段呈现RFLP多态性,有AA、AB、BB3种基因型,A基因频率较高。相关分析结果表明,不同基因型的产蛋数差异不显著。[结论]A基因可能是影响产蛋数的优势基因。
[Objective] The study aimed to analyze the PRL gene polymorphism of Jinshui silk fowl, and accumulating molecule basic data for protecting genetic resources and breeding afterwards and improving performance. [Method] The Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms （PCR-RFLPs） of PRL gene in 165 Jinshui silk fowls were investigated. Two restrictions of Hha Ⅰ, Hoe Ⅲwere used to digest the production of PCR respectively. [Result] It was demonstrated that 3 different genotypes of AA,AB,BB had been detected for Hoe Ⅲ site. The frequency of A gene was greatly higher than that of B gene. The result of relation test showed that there was no significant difference for laying performance between the chicken of different genotypes. [Conclusion] A gene could be the superiority gene of effecting the laying performance.
出处
《安徽农业科学》
CAS
北大核心
2008年第11期4457-4458,4597,共3页
Journal of Anhui Agricultural Sciences
基金
湖北省自然科学基金项目(2005ABA194)
湖北省动物胚胎工程及分子育种重点实验室项目(2007A0701)
作者简介
潘爱銮（1972-），女，瑶族，湖南江华人，硕士，助理研究员，从事家禽育种方面的研究。
通讯作者。
