摘要
探讨树莓酮对高脂饮食引起的非酒精性脂肪性肝炎作用机制。雄性SD大鼠60只随机分为5组。对照组普通饲料喂养;模型Ⅰ、Ⅱ组喂高脂饲料;树莓酮Ⅰ、Ⅱ组分别在喂饲高脂饲料4周后予1%树莓酮灌胃。8周末处死对照组、模型Ⅰ组和树莓酮Ⅰ组大鼠,模型Ⅱ组、树莓酮Ⅱ组大鼠继续喂养至12周末处死,测定大鼠血清转氨酶(ALT、AST)、血脂(TC、TG)、血糖(GLU)、胰岛素(INS)、胰岛素敏感指数(ISI)、瘦素(LEP)和肿瘤坏死因子α(TNFα)水平,测定肝匀浆MDA含量和SOD活性,免疫组化法测肝PPARγ表达。与对照组比较,模型Ⅰ、Ⅱ组ALT、AST、TG、TC显著增高(P<0.05,P<0.01),血糖。胰岛素水平显著增高(P<0.01)。模型组和树莓酮组胰岛素敏感指数显著下降(P<0.05,P<0.01),模型组肝MDA含量和血清瘦素,TNFα水平显著增高,SOD活性下降明显(P<0.05,P<0.01),树莓酮组血清TNFα显著增高(P<0.01);模型组PPARγ阳性表达细胞明显减少(P<0.05)。与模型Ⅰ、Ⅱ组比较,树莓酮Ⅰ、Ⅱ组对上述指标均显著改善(P<0.05,P<0.01)。可见树莓酮可通过调节糖脂代谢紊乱,改善瘦素抵抗,抗脂质过氧化和抑制炎症反应等综合作用来治疗非酒精性脂肪性肝炎,其机制可能与增强PPARγ表达有关。
To explore the mechanisms of raspberry ketone in the treatment of steatohepatitis in rats fed by highfat diets. Sixty male Sprague Dawley rats were randomly assigned into 5 groups. The rats fed by normal food served as the controls. The rats in model I, II group were fed with high-fat diets, and those in raspberry ketone Ⅰ, Ilgroup were given 1% raspberry ketone 4 w after high-fat diets feeding respectively. The rats in control, model Ⅰ and raspberry ketone Ⅰ group were killed at 8 w and the rest ones were killed at 12 w. Blood sample were collected for the detection the levels of aspartate transaminase （AST）, alanine aminotransferase（ALT）, cholesterol（TC）, glycerinate（TG）, glucose（GLU）, insulin（Ins）, insulin sensitivity index（ISI）,leptin（LEP） and tumor necrosis factor alpha（TNFα.and liver tissues were obtained for the detection of malondialdehyde （MDA） contents, superoxide dismutase （SOD） activity and PPARγ is detected by immuneohistochemistry. Results In comparison with those of control rats, the levels of ALT, AST, TG, TC and TG and TC were increased markedly （P〈0.05,P〈0.01） and the contents of MDA and the levels of LEP and TNFα were increased notablely （P〈0.05,P〈0.01）, and insulin sensitivity index and the SOD activity were decreased significantly （P〈0.05,P〈0.01） in model Ⅰ, Ⅱ group, and insulin sensitivity index were decreased （P〈0.05）in raspberry ketone Ⅰ, Ⅱ group and the levels of TNFtt were increased significantly （P〈0.01） in model Ⅱ group, and the number of PPARγ immunostaining positive expression cells were decreased significantly （P〈0.05）. In comparison with those of model rats, the markers such as ALT, AST, MDA, SOD, TNFα and PPARγ etc. were all significantly improved in the raspberry ketone group. Raspberry ketone has the action of regulating glucolipid metabolic disorder, ameliorating insulin resistance, anti-lipid peroxidation, and anti-inflammation, and its mechanism may be related to decreased enhanced PPARγ expression.
出处
《食品工业》
北大核心
2008年第2期6-9,共4页
The Food Industry
