摘要
Al2O3作核心的脱乙酰几丁质颗粒可用作酶固定化的载体。观察了脱乙酰几丁质的浓度、脱乙酰度和分子量对酶的固定化的影响。发现以浓度为2．0％、脱乙酰度为85．0％、分子量为1．34×106d的脱乙酰几丁质制得的载体用于固定化酶效果最好。所得固定化GOD具有较高的固定化酶活力、热稳定性和工作稳定性。
In the present study chitosan-coating Al2O3 was used as supports for enzyme immobilization. It was discovered that different concentrations of Al2O3-chitosan, degrees of deacetylation and molecular weights of chitosan were related to the immobilization. When Al2O3-chitosan was at concentration of 2. 0%, deacetylation at 85% and with a molecular weight of 1 .03 × 106 Dalton, the best immobilization was obtained. The immobilized GOD showed a high enzymatic activity, improved thermal stability and longer operating stability.
出处
《蚕业科学》
CAS
CSCD
1997年第3期158-162,共5页
ACTA SERICOLOGICA SINICA
