摘要
多酶共固定体系以级联反应体系为基础,将两种或两种以上的酶固定在同一载体上,具有较高的原子经济性和可持续利用性,已成为材料科学、生命科学、生物医药等多种领域的研究热点。选择适宜的载体材料是提高多酶共固定体系催化效率最基本也是最重要的途径。本文以葡萄糖氧化酶和辣根过氧化物酶构建的级联反应作为研究对象,分别从随机固定、分区域固定以及定向固定这三种不同的载体和酶之间的作用形式入手,概述了目前用于多酶共固定体系的载体研究进展。最后分析了多酶共固定领域目前存在的局限和挑战,以期为更多多酶共固定体系提供研究思路。
Multi-enzyme co-immobilization systems,in which two or more enzymes are immobilized onto or into the same carrier,are based on enzyme cascade reactions.Due to their high atomic economy and sustainable utilization properties,such systems have become a research hotspot in various fields such as material science,life science,and biomedicine.Selecting suitable carrier materials is the most basic and important way to improve the catalytic efficiency of multi-enzyme co-immobilization system.In this paper,the cascade reaction consisted of glucose oxidase and horseradish peroxidase was used as the model reaction,and the current research progress of carriers for multi-enzyme co-immobilization was summarized from three different interaction forms between carriers and enzymes:random immobilization,partition immobilization and directional immobilization,respectively.Finally,in order to provide research ideas for more multi-enzyme co-immobilization systems,the limitations and challenges in this field were analyzed.
作者
张耀丹
孙若溪
陈鹏程
ZHANG Yaodan;SUN Ruoxi;CHEN Pengcheng(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China)
出处
《化工进展》
EI
CAS
CSCD
北大核心
2023年第6期3167-3176,共10页
Chemical Industry and Engineering Progress
基金
江南大学2021年本科教育教学改革研究项目(JG2021056)。
关键词
级联反应
酶固定化
多酶共固定化
载体材料
cascade reactions
enzyme immobilization
multi-enzyme co-immobilization
carrier material
作者简介
第一作者:张耀丹(1995-),女,硕士研究生,研究方向为生物与医药。E-mail:zhangyaodanjy@163.com;通信作者:陈鹏程,副教授,研究生导师,研究方向为轻工技术与工程。E-mail:chenpengcheng@jiangnan.edu.cn。
