摘要
背景与目的:去甲斑蝥素(norcantharidin,NCTD)具有抑瘤作用,本文研究它对血管生成的作用,以及对人乳腺癌MCF-7细胞鸡胚绒毛尿囊膜(chorioallantoci membrane,CAM)移植肿瘤血管生成的影响。方法:①建立鸡胚绒毛尿囊膜模型,将明胶海绵载体置于两条前卵黄静脉间血管的CAM上,将不同剂量的NCTD加在载体上,以生理盐水对照,观察记录载体周围血管分支点数,计算血管生成抑制率。②建立人乳腺癌MCF-7细胞鸡胚移植模型。给予不同剂量的NCTD20μl[72、36、18μg/(胚.d)],以生理盐水对照,计算血管生成抑制率。结果:NCTD对以明胶海绵为载体的鸡胚绒毛尿囊膜血管生成具抑制作用,72、36、18、9μg NCTD组血管生成抑制率分别为77.7%、62.9%、50.6%及33.0%,并且呈剂量依赖性。移植肿瘤可诱发血管生成,NCTD能明显抑制MCF-7鸡胚移植肿瘤血管生成,72、36、18μg NCTD对移植瘤诱导的血管抑制率分别为66.2%、39.3%和22.8%。结论:NCTD能明显抑制鸡胚绒毛尿囊膜新生血管的生成,同时能抑制人乳腺癌MCF-7细胞鸡胚移植肿瘤的血管生成。其抗肿瘤作用与抑制肿瘤血管生成有关。
Background and purpose：Norcantharidin（NCTD）can inhibit the growth of tumors.In this study,we presented the chorioallantoci membrane（CAM）model to evaluate the effect of NTCD on angiogenesis and to study the anti-angiogenesis of NTCD in the chicken embro implant model of human breast carcinoma MCF-7 cells.Methods：The method was based on the implantation of gelatin sponges on top of growing CAM.The sponges were treated with various amounts of NCTD and 0.9% NaCl.Blood vessels surrounding CAM mesenchyme were counted.Then we established the MCF-7 chicken embryo implant model.The chicken embryo CAMs was treated with 0.9% NaCl,Various amounts of NCTD（72,36,18 μg per 20 μl）and the inhibition rates were calculated.Results：NCTD can inhibit the new capillary vessels growing into gelatin sponges placed on the CAM in a dose-dependent manner,and the inhibitory rates were 77.7%,62.9%,50.6% and 33.0% respectively.NCTD at the dosages of 72、36、18 μg were able to inhibit capillary growth regardless of the angiogenic process induced by xenograft tumor and had a significant inhibition as compared with the control group,and the rates were 66.2%,39.3%,22.8% respectively.Conclusions：NCTD has an anti-angiogenic effect for targeting tumor angiogenesis.NCTD may be a potential therapeutic candidate for clinical application.
出处
《中国癌症杂志》
CAS
CSCD
2007年第11期847-850,共4页
China Oncology
关键词
去甲斑蝥素
鸡胚绒毛尿囊膜
血管生成
抗血管生成
norcantharidin
chick embryo chorioallantoic membrane
angiogenesis
anti-angiogenesis
作者简介
通讯作者：林晓燕E-mail：1xy@tocc．net
