摘要
目的:研究桑黄菌原生质体分离与再生的条件。方法:研究以菌丝体为材料的不同酶解条件对桑黄菌原生质体产量的影响及不同再生条件对桑黄菌原生质体再生率的影响。结果:在1.5%溶壁酶+0.5%崩溃酶的混合酶系、酶解温度为30℃、菌龄为8 d、蔗糖为酶解渗透压稳定剂、酶解时间为3 h的条件下,桑黄菌原生质体分离产量较高,但兼顾菌丝量和原生质体的再生,较适宜的菌龄为10 d,较适宜的酶解渗透压稳定剂是甘露醇。另外用甘露醇作为培养基渗透压稳定剂、用加桑枝的马铃薯葡萄糖再生培养基进行再生,桑黄菌原生质体再生率较高。结论:在综合考虑桑黄菌原生质体的产量与再生的前提下,获得了使桑黄菌原生质体产量与再生率均较高的试验条件。
Objective： To study the conditions on separation and regeneration of protoplast from Phellinus igniarius. Method： The effects of enzymolysis conditions of P. igniarius mycelia on yield of protoplast and culturing conditons on regeneration ratio of protoplast were investigated. Result： When the 8 days-old mycelia was hydrolysed by 1.5% of lywallzyme adding to driselase of 0. 5% and at 30℃ for 3 h and enzymolysis was stablized by sucrose as a stablisher of osmotic pressure, higher yield of P. igniarius protoplast was obtained. If 10 days-old mycelia was used as raw material of enzymolysis and manntol was selected as stablisher of osmotic pressure of enzymolysis, higher regeneration ratio of P. igniarius protoplast also would be obtained in following regeneration step at same time keeping higher yield. For the regeneration processing, it was beneficial for the regeneration of P. igniarius protoplast that PDA plusing mulberry ramulus was used as the culture medium of regeneration and manntol was selected as the osmotic pressure stablisher of regeneration culture medium. Conclusion： The method and conditions to keep both higher yield and regeneration ratio of P. igniarius protoplast were obtained.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第21期2232-2235,共4页
China Journal of Chinese Materia Medica
关键词
桑黄菌
原生质体
分离
再生
Phellinus igniarius
protoplast
separation
regeneration
作者简介
[通讯作者]马海乐，Tel：（0511）8780174．E-mail：mhl@．is．edu．cn
