摘要
从活性污泥中筛选出一株产絮凝剂的霉菌,初步鉴定为黑曲霉(Aspergillus niger)。其所产生的微生物絮凝剂对高岭土悬浊液具有良好的絮凝作用。通过培养条件优化,其所产生的微生物絮凝剂对高岭土悬浊液的絮凝率从85.6%提高到98.7%,实验结果表明,(1)黑曲霉孢子最佳接种量为9.33×108个/L;(2)产絮凝剂适宜的碳源为蔗糖,氮源为尿素,用量分别为30.00、1.600g/L;(3)最佳培养条件为:查氏液体培养基初始pH7.0,培养温度30℃,摇床转速180r/min,培养时间120h;(4)在最佳培养条件下微生物絮凝剂的产量为1.805g/L;(5)絮凝性能比较结果表明,微生物絮凝剂的絮凝性能优于传统絮凝剂。
A flocculant-producing strain, Aspergillus niger was isolated from the activated sludge sample of a domestic wastewater treatment plant. The bioflocculant was effective in reducing turbidity of a kaolin clay suspension. The bioflocculant produced under the optimized culturing conditions was even more effective; its flocculation activity for the kaolin clay suspension increased from 85.6% to 98.7%. The bioflocculant production was 1. 805 g/L under the optimum culturing conditions： inoculation density= 9. 33×10^8 L^-1 , source of earbon= sucrose （30. 00 g/L）, source of nitrogen=urea （1. 600 g/L）, initial pH=7.0, temperature=30℃, the rotation speed= 180 r/min, and culturing timex 120 h. The bioflocculant was more effective than 2 popular organic and inorganic flocculants.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2007年第10期735-739,共5页
Environmental Pollution & Control
基金
江苏大学高级人才基金资助项目(No04JDG017)
关键词
微生物絮凝剂
黑曲霉
条件优化
Bioflocculant Aspergillus niger Optimization of culturing conditions
作者简介
刘彬彬，女，1982年生，硕士研究生，研究方向为微生物絮凝剂的制备及应用。
