摘要
从活性污泥中分离筛选到一株产絮凝剂的细菌A2 5 ,鉴定为巨大芽孢杆菌 (Bacillusmegaterium) .A2 5产生絮凝剂与菌体生长同步 ,均在 10h达到最高值 .用乙醇沉淀及SephacylS - 5 0 0分子筛层析得到纯化的絮凝剂BP2 5 .通过Bradford反应、琼脂糖凝胶电泳及硫酸 -酚法测定糖 ,证明BP2 5是一类多糖类物质 .其分子量为 10 6道尔顿 .薄层层析的结果表明BP2 5含有葡萄糖和甘露糖两种单糖 ,高效液相色谱分析其摩尔比为 4∶1.实验证明 ,BP2
A bacterial strain A25,isolated from activated sludge and identified as Bacillius megaterium, can produce flocculant.The flocculant was synthesized during the cell growth.The purification of flocculating substance was carried out by ethanol precipitation and Sephacry S-500 chromatography methods.The purified flocculant BP25 was assayed by Bradford reaction,agarose gel electrophoresis and Sulfate-phenol method.The results showed that BP25 is a kind of polysaccharide with molecular weight of 10°daltons by...
出处
《环境科学学报》
CAS
CSCD
北大核心
2001年第S1期47-52,共6页
Acta Scientiae Circumstantiae
基金
九五’攻关项目 (项目编号 :96 - 90 9- 0 1- 0 3)
关键词
巨大芽孢杆菌
絮凝及絮凝剂
污水处理
Bacillus megaterium
flocculate and flocculant
treatment of wastewater
