摘要
目的观察益生菌制剂对急性肝内胆汁淤积幼鼠胃肠消化间期移行性肌电复合波（MMC）影响,探讨其干预胆汁淤积的可能机制。方法SD幼鼠96只随机分为健康对照组16只,中毒组、干预组各40只。各组随机选出8只在胃窦、十二指肠、空肠埋置3对银丝电极,余大鼠行假手术。术后7-10 d中毒及干预组1次灌服异硫氰酸萘酯（ANIT）（200 mg/kg）诱导急性肝内胆汁淤积;干预组于ANIT灌胃前2 d,灌服双歧三联活菌片4.2×10^8活菌/（kg.d）。观察各组灌服ANIT后48、96、144、192 h胆汁流量、血总胆红素（TB）、ALT及MMC变化。结果1.灌服ANIT后,干预组胆汁流量减少程度及血清中TB和ALT上升较中毒组轻,且恢复快。2.灌服ANIT后中毒及干预组MMC节律完全消失,代之以Ⅱ期样节律紊乱波;随后2组MMC逐渐恢复,但干预组MMC恢复时间（132.0±42.55）h明显短于中毒组（174.0±24.84）h（P〈0.05）;MMC节律恢复后,192 h时中毒组MMC持续时间较干预及健康对照组明显延长,其中主要是Ⅱ期持续时间延长。结论幼鼠急性肝内胆汁淤积时MMC周期延长,甚至节律性运动消失。双歧三联活菌片能促进肠道MMC节律性运动恢复,减轻肝脏损害,有助于改善胆汁淤积。
Objective To investigate effect and mechanism of probiotic products on α - naphthylisothiocyanate（ANIT） induced intrahepatic cholestasis of weaned rats through the changes of gastrointestine myoelectric migrating complexes（MMC）. Methods Ninety - six SD rats were randomly divided into 3 groups：control group（n = 16） ,intoxication group（ n =40） and intervention group（n =40）. Three pairs of bipolar silver electrodes were chronically implanted in the antrum, duodenum and jejunum of 8 rats from every group. Sham operation was given to the others of the 3 groups. Seven to 10 days after operation, all rats of intoxication and intervention groups received a single intragastric administration of ANIT（200 mg/kg） in order to induce acute intrahepatic cholestasis. Probiotic products,4.2 ×10^8/（ kg·d） ,was given to intervention group 2 days before ANIT was administrated. The gastrointestinal MMC was recorded in conscious and fasting state every 48 h till 192 h after ANIT was given. Biliary flow, total bilirubin（TB） and ALT was recorded in sham operated rats at the same time. Results 1. Forty - eight hours after ANIT was garaged, the biliary flow of intoxication and intervention groups decreased while ALT and TB increased. But the above observed indexes of intoxication group were more significantly altered than those of intervention group. 2. A typical MMC pattern could be seen in all normal conscious fasting rats and it disappeared temporarily after ANIT was given. Ninety - six to 192 h later, the MMC pattern recovered gradually and intervention group＇s MMC pattern appeared earlier than that of the intoxication group. But increased MMC cycle duration was seen in the intoxication group at 192 h and it was characterized by an increased duration of phase Ⅱ - like activity. Conclusions MMC pattern disappeared or the duration of MMC become longer when acute cholestasis was induced with ANIT in rats. Probiotic products can promote the initiation of MMC and decrease the cycle duration, thereby relieve the cholestasis.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第19期1487-1489,共3页
Journal of Applied Clinical Pediatrics
关键词
消化间期
肌电复合波
移行
胆汁淤积
肝内
益生菌
interdigestive
myoelectric complexe, migrating
cholestasis, intrahepatic
probiotics
作者简介
胡玉莲，女，副主任医师，博士生，研究方向为消化系统疾病。
通讯作者：黄志华，男，主任医师，教授，博士生导师，研究方向为消化系统疾病。
