摘要
目的:探讨丹参酮ⅡA对HeLa宫颈癌细胞凋亡的影响及其作用机制。方法:应用MTT比色法检测不同浓度丹参酮ⅡA对HeLa宫颈癌细胞的增殖抑制,采用烟酸己可碱33258染色法观察丹参酮ⅡA对HeLa细胞凋亡的影响,并用半定量逆转录聚合酶链反应法检测用药后Bcl-2和bax基因mRNA的表达水平。结果:丹参酮ⅡA对HeLa细胞增殖的抑制作用呈剂量依赖性;它能促使HeLa细胞发生凋亡;用药48h后Bcl-2基因mRNA的表达水平明显降低,而bax基因的表达则无明显变化。结论:丹参酮ⅡA对HeLa细胞的增殖具有显著的抑制及诱导凋亡作用。
OBJECTIVE： To discuss the effect of tanshinone ⅡA on the apoptosis of HeLa cell line and its mechanism. METHODS： MTT was used to measure the levels of the proliferation of HeLa cells cultured with tanshinone ⅡA in different concentrations. The apoptosis was measured by Hoechst33258 staining, and the level of mRNA of Bcl-2 and bax in HeLa cells was tested by semi-fix quantify RT-PCR method. RESULTS： Tanshinone ⅡA could inhibit the proliferation of HeLa cells in a dose dependent manner, induce cell apoptosis, and markedly decrease the expression of Bcb2 on mRNA level after treatment for 48h, while it had no obvious change in bax mRNA level. CONCLUSION： Tanshinone ⅡA can inhibit the proliferation of HeLa cells and induce the apoptosis of the cells.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第27期2102-2104,共3页
China Pharmacy
作者简介
主治医师，硕士。研究方向：恶性肿瘤的防治。电话：0532-82828423。E-mail：shenjun1975@sohu.com
通讯作者：教授，博士研究生导师，研究方向：肿瘤生物治疗。电话：0532-82991209。E-mail：gcyinlin@126．com
