摘要
目的 :探讨丹参酮ⅡA对人胶质瘤细胞株U2 5 1的增殖抑制作用及其作用机理。方法 :应用MTT比色法检测不同浓度丹参酮ⅡA对U2 5 1细胞的增殖抑制 ,应用流式细胞仪观察丹参酮ⅡA对U2 5 1细胞周期的影响 ,应用RT -PCR观察相关基因C -myc表达的变化。结果 :丹参酮ⅡA对U2 5 1细胞增殖抑制作用呈剂量依赖性 ,当浓度为 0 10g·L-1时 ,对U2 5 1细胞增殖的抑制率达到 (5 3 7± 6 0 ) %。流式细胞仪分析显示 ,用 0 10g·L-1丹参酮ⅡA培养 3d ,细胞周期中G0 /G1期所占比例增高 ,S期占细胞周期的比例降低。RT -PCR结果显示 ,随着丹参酮ⅡA作用剂量的增加 ,c -myc基因的表达被明显抑制。结论 :丹参酮ⅡA对人胶质瘤细胞系U2 5 1的增殖具有明显的抑制作用 ,并对原癌基因c -myc的表达具有抑制作用。
AIM: To investigate the inhibitory effect of TanshinoneⅡA on U251 glioma cell line and its mechanism. METHODS: MTT was used to measure the levels of the proliferation in U251 cultured with TanshinoneⅡA at different concentrations. The effects of TanshinoneⅡA on cell cycle of U251 were observed by FCM. The expression of proto-oncogene c-myc was measured by RT-PCR. RESULTS: The proliferation of U251 was obviously inhibited by TanshinoneⅡA in a dose dependent manner. The inhibitory rate came to the peak at (54 2±0 9)%, when cultured with TanshinoneⅡA at 0 10 g/L. The outcome of FCM showed that the proportion of G 0/G 1 phase cells was increased and the proportion of S phase cells was reduced obviously, when cultured with TanshinoneⅡA at 0 10 g/L for 3 days. The RT-PCR experiment showed that the expression of proto-oncogene c-myc was notably decreased, when the dose of TanshinoneⅡA was increased. CONCLUSION: TanshinoneⅡA inhibited the proliferation of U251 and the expression of proto-oncogene c-myc.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第6期981-984,共4页
Chinese Journal of Pathophysiology
