摘要
从乌拉尔甘草健康植株的根茎叶中共分离到内生细菌98株,经初步鉴定芽孢杆菌属(Bacillus sp.)为优势种群,约占30%;从不同生长年份甘草的根、茎、叶组织中分离内生细菌种群密度从5.0×104cfu/g^2.9×107cfu/g鲜重不等。采用平板对峙方法筛选出6株对植物病原菌有明显体外拮抗活性的菌株,通过菌落、菌体形态观察、生理生化反应及16S rDNA序列分析,同时结合Biolog细菌自动鉴定系统验证,鉴定这6株拮抗菌分属萎缩芽孢杆菌(Bacillus atrophaeus)、多粘类芽孢杆菌(Paenibacillus polymyxa)、枯草芽孢杆菌(Bacillus subtilis)、Paenibacillus ehimensis。
98 endophytic bacteria strains were isolated from different internal tissues of Gtycyrrhiza uralensis plants collected from lnnennongolia region. Results indicated that the population densities of endophytic bacteria ranged from 5.0 × 10^4 cfu/g - 2.9 × 10^7 cfu/g fresh weight although it varied depending on tissue of the plant. Among these strains, Bacillus sp. was the most prevalent endophytic bacterium, which was amount to 30%. Of the 98 isolates, 6 strains exhibited extensive antagonistic activities against pathogenic bacteria. Characterization showed that these bacteria were Bacillus atrophaeus, Paenibacillus polymyxa, Bacillus subtilis, Paenibacillus ehimensis. This study indicated that selected 6 endophytic bacteria strains have potential for biological control of plant disease.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第4期700-704,共5页
Microbiology China
基金
国家973计划资助项目(No.001CB1089)
国家自然科学基金资助项目(No.30672616)
中央级公益性科研院所基本科研业务费专项资金
关键词
甘草
内生细菌
抗菌活性
芽孢杆菌
鉴定
Glycyrrhiza uralensis, Endophytic bacteria, Antagonistic bacteria, Bacillus sp., Identify
作者简介
通讯作者Tel：010-68975891，E—mail：dlshen@caas．ac．cn
