摘要
从乌拉尔甘草样品的根、茎中共分离出54株内生真菌,统计发现从根中分离得到的内生真菌的种类比茎中多。抑菌实验共得到20株活性菌株,其中编号为dG9-1的菌株对多种病原菌有抗菌活性。通过进一步对其培养特征,显微形态特征观察以及ITS序列测定和系统发育树构建与分析,发现该菌与灰黄青霉(Penicillium griseofulvum)的亲缘关系最接近,相似性为99.46%。
Fifty-four endophytic fungus were isolated from roots, stems of Glycyrrhiza uralensis. Twenty strains were obtained by the inhibitory activity screening and dGg-1 had the best antimicrobial function among them. Through colony characteristics and morpho- logical characters by microscopy and ITS sequence, analysis results showed that the strain dG9-1 was similar to Penicillium griseofulvum, and the similarities was 99. 46%.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第6期155-158,共4页
Biotechnology Bulletin
基金
北京市教委基金(KM200510082008)
关键词
乌拉尔甘草
内生真菌
抑菌活性
鉴定
ITS序列
Glycyrrhiza uralensis Andophytic fungus Antimicrobe activity Identification ITS sequence
作者简介
王丽（1981-），女，硕士生，主要从事植物内生菌的研究；E-mail：laiyang1981@163．com
通讯作者：韩素贞，E-mail：hansuzhen99@vip．sina.com
