摘要
通过对茶树花物料粒度、不同提取剂和提取方式的比较,研究茶树花黄酮的最佳提取工艺;并比较不同提取方法获得的茶树花黄酮提取物对羟自由基(.OH)的清除效果.结果表明:乙醇热回流提取的最佳工艺条件为:体积分数95%的乙醇,料液比1∶15,80℃下批次提取90 min;超声波振荡提取最佳工艺为:体积分数95%的乙醇,料液比1∶30,提取温度45℃,批次提取80 min.超声波结合热提法获得的茶树花黄酮提取物对.OH的清除效果最好.
The optimal extraction process of Tea Flower flavonoids was studied by investigating different particle sizes, extraction reagents and methods. Comparisons were also made among the effects of flavonoids extracted by different methods on scavenging hydroxyl radicals. Results showed that ： （ 1 ） the optimal conditions of heat extraction method were ： solid-liquid ratio was 1 ： 15, extraction temperature was 80℃, and volume fractions of ethanol was 95% , with 90 minutes each time; （2） optimal conditions of ultrasonic extraction method were： solid-liquid ratio was 1：30, 45℃ , and volume fractions of ethanol was 95%, with 80 minutes each time. Results also showed that Tea Flower flavonoids extracted by ultrasonic combined with heat extraction had the highest scavenging activity on hydroxyl radicals.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期93-97,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
国家十五科技攻关课题(2001BA502B09)资助项目
关键词
茶树花
黄酮
提取
清除
羟自由基
Tea Flower flavonoids, extraction, scavenge, hydroxyl radicals
作者简介
陈小萍（1981-），女，硕士研究生，主要从事农产品加工与贮藏的学习与研究．E—mail：chenxp_81@163．com
通讯联系人：张卫明（1957-），教授，博士生导师，主要从事野生资源开发与利用的教学与研究．E—mail：botanyzh@163．com
