摘要
本试验采用波兰黑穗醋栗(Ribes nigrumL.)优良品种Ceres冬季休眠枝的水培芽为外植体,进行了组织培养研究,建立了其快繁体系,即初代培养基为MS+6-BA 1.0 mg/L;增殖培养基为MS+6-BA 0.5 mg/L+IBA 0.5mg/L;生根培养基为MS+无激素;在此体系中增殖系数可达4.3,生根率100%,最多生根条数可达15条以上。本试验还发现,Ceres品种茎段分枝力较差,但生根能力很强。
The buds from water cultured dormant branches of Ribes nigrum L.cv.Ceres were taken as explants in this experiment. The optimum media for initiating, proliferating, and roofing culture were established, i.e., MS＋ 6- BA 1.0 mg/L,MS ＋6-BA 0.5 mg/L ＋ IBA 0.5 mg/L and MS＋ no hormone,respectivdy. In this system, the highest propagation coefficient was 4.3 and the rooting rate was 100%, more than 15 roots were formed in rooting medium. The result also indicated that Ceres has low branching ability and high rooting ability.
出处
《河北林果研究》
2006年第4期419-421,共3页
Hebei Journal of Forestry and Orchard Research
基金
中波政府间科技合作项目(AM30-033)
作者简介
赵锦（1977-），女，河北安平人，博士，副教授，从事植物种质资源研究。
通讯作者：刘孟军（1965-）。男，河北望都人，教授，从事植物种质资源和育种研究。
