摘要
目的比较铁皮石斛和金钗石斛在化学成分上的差别。方法用高效液相色谱-质谱联用(HPLC/MS)法,比较铁皮石斛和金钗石斛氨性氯仿提取物中各成分的色谱峰相对积分面积和含量。结果铁皮石斛和金钗石斛中电喷雾电离质谱质量数为偶数的色谱峰的相对积分面积和分别为2·34%和41·87%。铁皮石斛和金钗石斛中共有的25个相同的化学成分,相对积分面积和分别为97·12%和50·09%,其中有23个成分在铁皮石斛中的含量高于金钗石斛。结论铁皮石斛和金钗石斛在生物碱类成分的数量和含量上有很大差别,但就所含的相同化学成分而言,铁皮石斛的质量好于金钗石斛。
Objective To compare the chemical compositions between Dendrobium candidum (D. candidum) and Dendrobium nobile ( D. nobile). Method Relative area and content of every chromatographic peak in the ammonic chloroform extracts of D. candidum were compared with those of D. nobile with high performance liquid chromatography-mass spectrometry. Results The relative area of alkaloids accounted for 2.34% and 41.87% in D. candidum and D. nobile, respectively. The relative area of 25 identical compositions took up 97.12% in the total area of D. candidum and 50.09% in that of D. nobile, and contents of 23 composition were higher in D. candidum than those in D. nobile. Conclusions The quantity and contents of alkaloids are remarkably different between D. candidum and D. nobile. However, D, candidum has a higher quality than D. nobile in terms of the same chemical compositions.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2006年第4期524-529,共6页
Acta Academiae Medicinae Sinicae
基金
国家杰出青年科学基金专项(30325047)~~
关键词
铁皮石斛
金钗石斛
高效液相色谱-质谱联用法
化学成分
Dendrobium candidum
Dendrobium nobile
high performance liquid chromatography-mass spectrometry
chemical composition
作者简介
Corresponding author Tel :010-62828619, Fax : 010-62895120, E-mail : sxguo2006@yahoo.com.cn