摘要
目的通过无菌茎段实现铁皮石斛快速繁殖。方法将铁皮石斛试管苗茎节接种于不同NAA浓度的MS培养基上诱导腋芽萌发,并在含不同激素配比和有机添加物的培养基上继代和诱导生根。结果NAA0.4mg/L,6-BA5mg/L时铁皮石斛腋芽萌发率达93.33%;NAA0.3mg/L,6-BA2mg/L时丛苗的生根率达96.67%,香蕉汁和土豆汁能够显著促进丛生芽的增殖和壮苗生根。结论建立了适合铁皮石斛茎段离体培养的快速繁殖体系。
Objective To develop a rapid propagation of Dendrobium candidum using the aseptic nodal segments of shoots. Methods Axenic nodal segments were first germinated on Murashige and Skoog（MS） basal medium supplemented with different concentration NAA. The seedlings were subcultured and rooted on the medium containing different concentration hormones and organic susbstance. Results 93.33% of the axenic nodal segments of Dendrobium candidum germinated on the medium with NAA 0.4 mg/L,6 - BA 5 mg/L and 96.67% of explant rooted on the medium containing NAA 0.3 mg/L,6 - BA 2 mg/L. The growth of explant was promoted by the extract of banana and potato. Conclusion The in vitro propagation of Dendrobium candidum from axenic nodal segment has been established.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第11期2659-2660,共2页
Lishizhen Medicine and Materia Medica Research
基金
辽宁省农业生物技术重点实验室专项基金资助项目
关键词
铁皮石斛
无菌茎节
离体培养
激素浓度
有机添加物
Dendrobium candidum
Axenic nodal segments
In vitro propagation
Concentration of hormone
Organic susbstance
作者简介
郭洪波（1979-），男（汉族），山东聊城人，现为沈阳农业大学在读硕士研究生，主要从事生物工程制药研究工作．
通讯作者简介：陈丽静（1971-），女（汉族），山东海阳人，现任沈阳农业大学副教授，博士学位，主要从事植物细胞工程与基因工程研究工作．
