摘要
目的:研究10%四氯化碳致大鼠慢性肝损伤中间病理变化,为建立10%四氯化碳慢性肝损伤模型提供实验依据。方法:雄性Wistar大鼠80只,随机分为正常对照组和实验组,每组动物各40只。正常对照组腹腔注射玉米油,0.1 ml/100g.bw;实验组腹腔注射10%四氯化碳,0.1 ml/100g.bw。于实验期的第1、3、6和8周,两组动物分别腹腔注射玉米油和四氯化碳后48 h放血处死,取肝组织作组织病理学观察。结果:实验组染毒1周后,肝小叶中央静脉周围大量细胞脂肪变性,肝小叶中央区有少量细胞气球样变;实验组染毒3周后,肝小叶中央静脉周围大量细胞脂肪变性,肝小叶中央区有少量细胞气球样变;实验组染毒3周后,肝小叶中央静脉周围大量细胞脂肪变性,肝小叶中央区有少量细胞气球样变;实验组染毒3周后,肝小叶中央静脉周围肝细胞气球样变加重;实验组染毒6周后,肝小叶中央静脉周围开始出现少量细胞坏死;实验组染毒第8周后,出现肝纤维化,未达到肝硬化。结论:建议少用该模型鉴定药物和保健食品对慢性肝损伤的保护作用。
Objective： To observe pathological changes of liver induced by 1096 carbon tetrachloride in rats, and to make an annirnal model of it. Methods. Eighty Wistar rats were randomly decided into 2 groups. The samples of liver were taken at 48 hours alter poisoning with the hernatoxylin - eosin. Masson, Relculin stains and examined under light microsoope. Result： The first week, the hepatic cells occurred fatty degeneration and ballooning degeneration; in the sixth week, Necrotic hepatic cells around centric vein were observed; in the eighth week, the liver turned into Hepatic fibrosis. Conclusion： The chronic model in rats induced by 10% CCl4 should not be use to judge the positive effect of medicine and health food on chronic liver injury.
出处
《现代预防医学》
CAS
北大核心
2006年第5期701-703,共3页
Modern Preventive Medicine
作者简介
王宇，男（1980-），在读硕士研究生，毒理病理专业．
[通讯作者]程薇波。E-mail：marychen@mail．sc．cninfo．net
