摘要
目的研究薄荷油致小鼠肝毒性的量效、时效关系及自由基损伤机制。方法小鼠口服不同剂量薄荷油，测定其LD50。另取小鼠按不同剂量或不同时间点分组，检查血清ALT等肝功能指标和肝组织形态变化，测定肝组织SOD，MDA。结果小鼠口服薄荷油的LD50为3．0ml／kg。ALT等肝功能指标在给药后24—48h达到高峰，72h可恢复近正常值。与正常组相比，薄荷油中、高剂量组对肝组织损伤显著，随着剂量增大，ALT等肝功能指标升高显著（P〈0．05或P〈0．01）。薄荷油组与正常组比较，小鼠肝组织中SOD降低而MDA升高（P〈0．05），与四氯化碳相似。结论小鼠一次性口服大剂量薄荷油可造成急性肝损伤甚至死亡，并显示有毒性时-效、量-效关系；自由基损伤可能是其肝毒性机制之一。
Objective To study the time - dose effects and free radical mechanism of peppermint oil on liver damnification in mice. Methods The medial lethal dose（ LD50 ） of mice was determined when they took a different dose of peppermint oil orally. After other mice were divided into different groups according to different dose or time, hepatic functional parameters in serum were examined for example alanine aminotransferase（ALT） and so on. The morphologic change of hepatic tissues were observed, and superoxide dismutase（SOD） and malondialdehyde（MDA） of hepatic tissues were detected . Results The orally LD50 of peppermint oil was 3.0 ml/kg. Hepatic functional parameters in serum reached to the max at 24 - 48 hours after being intervened, which recovered close to normal values at 72 hours. Compared with that of the control group, hepatic tissues of the high and medium dose groups were damaged significantly, and hepatic functional parameters in serum advanced significantly （ P 〈 0.05 or P 〈 0. 01 ）. SOD of hepatic tissues of mice taking peppermint oil was low and MDA of that was high compared with that of the control group, which was similar to that of carbon tetrachloride. Conclusion Acute toxicity even death may occur when mice take a large dose of peppermint oil once orally. Time-dose-toxicity effects would be indicated. Free radical damnification might be one of the liver injury mechanism of peppermint oil.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第12期2954-2956,共3页
Lishizhen Medicine and Materia Medica Research
基金
上海市教委科研基金资助项目(No.05CZ06)
关键词
薄荷油
小鼠
肝毒性
时间-剂量-毒效
机理
Peppermint oil
Mouse
Liver damnification
Time-dose-toxicity effects
Mechanism
作者简介
刘红杰（1974-），男（汉族），山东邹平人，现任上海中医药大学讲师，博士研究生，硕士学位，主要从事中药药理毒理研究工作．
通讯作者简介：金若敏（1948-），女（汉族），上海人，现任上海中医药大学药物安全评价研究中心研究员，主要从事中药药理毒理研究工作．
