摘要
目的研究氯氰菊酯对小鼠肝组织的氧化损伤。方法昆明小鼠随机分为5组,包括1个阴性对照组(花生油)、3个氯氰菊酯染毒组(10、20和40 mg/kg)和1个高剂量氯氰菊酯(40 mg/kg)+维生素E(100 mg/kg)组,灌胃7 d,测定肝组织匀浆活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)的含量和肝组织细胞DNA-蛋白质交联(DPC)系数;并观察病理损伤。结果随着氯氰菊酯染毒剂量的升高,肝组织ROS、MDA、8-OHdG含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系,40 mg/kg组的上述指标与对照组差异均有统计学意义(P<0.05,P<0.01)。病理学观察可见40 mg/kg组肝细胞水肿严重,体积明显增大,胞浆疏松化甚至气球样变,肝索排列较紊乱。与单独氯氰菊酯染毒组相比较,40 mg/kg+VE组肝组织的ROS、MDA、8-OHdG含量和DPC系数均有下降,GSH含量上升(P<0.05,P<0.01)。结论 40mg/kg的氯氰菊酯能造成小鼠肝组织的氧化损伤和病理损伤,并可被维生素E所拮抗。
Objective To evaluate the oxidative damages induced by chlorpyrifos in mice liver tissue cells. Methods Kunming mice were randomly divided grouped into five groups： one solvent control group （peanut oil）, three cypermethrin groups （10, 20 and 40 mg/kg body weight group）, and one high dose cypermethrin （40 mg/kg） plus vitamin E （100 mg/kg） protection group. The mice were treated with the reagents through gavage daily for seven consecutive days. Afterwards, biomarkers including reactive oxygen species （ROS）, glutathione（GSH）, malondialdehyde（MDA）, 8-hydroxy-2-deoxyguanosine （8-OHdG） in liver tissues were determined. Results The contents of ROS, MDA, 8-OHdG and DPC coefficients of liver increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. There were significant differences in levels of each biomarker between 40 mg/kg group and control group （P〈0.05, P〈0.01）. The pathological examination showed that liver cellular swelling, the cytoplasm was inflated, some cells appeared ballooning change, and liver cords were disordered in 40 mg/kg cypermethrin group. Administration of vitamin E to cypermethrin-treated mice reflected that ROS, MDA , 8-OHdG and DPC decreased but GSH increased （P〈0.05,P〈0.01）. Conclusion Cypermethrin at certain doses （40 mg/kg） can induce oxidative stress and pathological damages in mice liver tissue, while vitamin E can protect against the damage induced by cypermethrin.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2014年第2期131-134,F0003,共5页
Journal of Environment and Health
基金
教育部国家级大学生创新训练计划项目(201210927036)
湖北省教育厅科学研究计划项目(D20122803)
作者简介
张忠杰（1991-），男，本科生，从事环境毒理学研究。
通讯作者：马萍，E-mail：mping68@126．com
