摘要
确定了双酶复合法提取制备大豆多肽的工艺条件。大豆蛋白经双酶复合酶解、活性炭脱色、超滤、真空浓缩和离心喷雾干燥等工艺,提取得到大豆多肽。通过试验确定,最佳复合酶解条件:中性蛋白酶与菠萝蛋白酶比例为3∶1、pH 7.0、底物浓度4.0%、酶解时间9 h;最佳脱色处理条件:粉末活性炭用量2.0%、温度50℃、pH 3.0、吸附时间3 h;超滤分离条件:NF-1纳滤膜、截留分子量2000 D、超滤压力1.0 mPa。
The preparation technology of soy peptides by double-enzyme composite hydrolysis was studied. Soy protein isolate was produced into soy peptides based on double-enzyme composite hydrolysis and purification technology. The result showed that the best composite enzymatic hydrolysis condition was： the composite enzymes were made up of neutral proteinase and bromelain with the proportion of 3 ： 1, pH value of 7.0, substrate concentration of 4.0 %, hydrolysis time of 9 h ; the best decoloration condition was ： quantity of activated carbon of 2.0 %, temperature of 50℃, pH value of 3.0, adsorption time of 3 h; the ultra-filtration condition was： NF-2 nanometer membrane, pressure of 1.0 mPa.
出处
《粮油食品科技》
2006年第2期22-24,共3页
Science and Technology of Cereals,Oils and Foods
基金
浙江省科技计划项目(2003C32022)
关键词
大豆多肽
双酶复合酶解
脱色
超滤
soy peptides
double-enzyme composite hydrolysis
decoloration
ultra-fihration
作者简介
周利亘（1966-），男，浙江绍兴人，硕士．
