摘要
以茎尖为外植体进行甜叶菊的组织培养,比以叶柄、叶片、茎段、下胚轴为外植体,愈仿组织诱导率、出芽率都高;在MS基本培养基上,适合茎尖生长、分化的激素浓度为0.5mg/LBA、0.5mg/LNAA;琼脂浓度为0.7%
Stem apex used as external plant.the higher induced rate of callus and the higher germi-nation rate could be obtained than petiole,leaf,Stem and hypocotyl used as external plant inStevia rebaudiana bertoni tissue culture. On MS minimal medium,suitable hormone concen-tration for stem apex growth and its differentiation were 0.5mg/L BA plus 0.5mg/L NAA,and the agar concentration wab 0.7%.
出处
《中国糖料》
1996年第3期24-26,共3页
Sugar Crops of China
关键词
甜叶菊
组织培养
外植体
激素
Stevia rebaudiana bertoni Tissuce culture External plant Hormone