摘要
以吕梁红枣为试材,采用复合酶提取法提取可溶性多糖,在单因素试验的基础上,通过正交实验法,优化了吕梁红枣多糖提取的最佳工艺条件;通过测定羟基自由基(·OH)的清除能力、DPPH自由基的清除能力、对菜籽油抗氧化性能,研究了红枣多糖的抗氧化作用,以期为红枣功能性产品的开发利用提供参考依据。结果表明:吕梁红枣多糖最佳提取工艺条件为木瓜蛋白酶浓度0.2%,果胶酶浓度1.0%,酶解温度45℃,酶解时间为90 min。伴随着红枣多糖浓度的逐渐增大,羟基自由基(·OH)的清除率、DPPH自由基的清除率、对菜籽油抗氧化性能均呈逐渐增大趋势,证实红枣多糖具有良好的抗氧化活性。该研究优化了吕梁红枣多糖提取工艺,并证实了红枣多糖具抗氧化性。
Taking Lyuliang jujube as the raw material,the soluble polysaccharides was extracted by complex enzyme extraction technique.On the basis of single factor experiment,the optimal process conditions for polysaccharide extraction of Lyuliang jujube were optimized by orthogonal test.The effect of antioxidant activity was evaluated by scavenging rate of·OH,DPPH free radical,and antioxidant effect on rap oil,in order to provide reference for the development and utilization of jujube functional products.The results showed that,when the papain concentration was 0.2%,pectase concentration was 1.0%,the temperature was 45℃,and the time was 90 minutes,the extraction was the best.With the polysaccharides concentration rasing,the scavenging rate of·OH,DPPH free radical,and antioxidant effect on rap oil were increased.It confirmed that jujube polysaccharides had a good antioxidant activity.This study was optimized polysaccharides extraction process.Antioxidant activity of polysaccharides was confirmed.
作者
呼凤兰
胡诗琪
HU Fenglan;HU Shiqi(Department of Life Sciences,Lyuliang University,Lyuliang,Shanxi 033000)
出处
《北方园艺》
CAS
北大核心
2022年第22期91-96,共6页
Northern Horticulture
基金
山西省高等学校科技创新资助项目(2020L0682)
山西省吕梁市科技局资助项目(2021NYGG-2-67)
关键词
红枣多糖
复合酶
提取
抗氧化性
red jujube polysaccharide
compound enzyme
extraction
antioxidant activity
作者简介
第一作者:呼凤兰(1980-),女,硕士,副教授,现主要从事食品加工与保藏等研究工作。E-mail:32491767@qq.com.
