摘要
刺五加（Ａｃａｎｔｈｏｐａｎａｘｓｅｎｔｉｃｏｓｕｓ）胚性愈伤组织经根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）Ｃ５８Ｃ１（ｐＢＺ６１１１）感染后，共培养４８～７２ｈ，再转移到含氯霉素５０ｍｇ／Ｌ的ＭＳ选择培养基（附加２，４－Ｄ０．５ｍｇ／Ｌ，ＮＡＡ０．２ｍｇ／ｌ）上继代培养５０～６０ｄ，筛选得到氯霉素抗性愈伤组织．该愈伤组织能在无激素的ＭＳ培养基上增殖，并检测到胭脂碱合成酶活性，表明是转化愈伤组织．转化愈伤组织可诱导形成胚状体，但胚状体分化形成的苗不能生根，可能是由于转化细胞内源细胞分裂素含量过高，抑制了根的分化．
in the report, we studied transformation of Acanthopanax senticosuscalli. The calli were infected with Agrobacterium tumefaciens strain C58C1 (pBZ6111) and cultured on MS sclective medium containing chloramphenical 50 mg/L,2, 4-D 0.5 mg/L and NAA 0. 2 mg/L to select transformants.Thechloramphenical-resistant calli were obtained when the Agrobacterium-infected calliwere subcultured on the MS selective medium for 50-60 days. The transformedcalli could grow on MS medium without phytohormone, and confirmed by analysisof nopaline synthetase(NOS)activity. Some embryoids from the transformed calligerminated and developed into shoots but non-rooted.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1995年第4期494-497,共4页
Journal of Central China Normal University：Natural Sciences
关键词
刺五加
愈伤组织
根癌农杆菌
遗传转化
中药
Acanthopanax senticosus
Agrobacterium tumefaciens
genetictransformation
