摘要
目的:筛选纯化白芍总苷的最佳树脂,确定树脂纯化白芍总苷的工艺参数。方法:以白芍中主要有效成分芍药苷的比吸附量、保留率、纯度为指标,对5种不同型号大孔吸附树脂进行筛选,确定了吸附分离白芍总苷的最佳树脂,并通过单因素考察确定了该树脂分离纯化白芍总苷的工艺条件。结果:AB8型树脂对白芍总苷有良好吸附分离性能,其吸附分离白芍总苷的工艺条件为:上样浓度为0.2g/ml,最大上样量为15g/ml树脂,吸附流速为3ml/min,洗脱剂为50%乙醇,洗脱流速为6ml/min,洗脱剂用量为5倍量树脂柱体积。结论:AB8型大孔吸附树脂在所确定的工艺条件下,纯化白芍总苷效果良好,芍药苷纯度可达46.49%。
Objective:To select the best macroporous resin and set up the method for purification of TGP.Methods:Five different types of macroporous resin were used to purify TGP. The adsorption ratio, reservation rate, purity were compared.Results:The AB-8 was the best for adsorbing and separating TGP in the following technological conditions: the sample concentration was 0.2 g·ml^(-1), the maximum capacity for medicinal materials was 15 g·ml^(-1), the current velocity was 3 ml·min^(-1), the eluting reagent was 50% ethanol(5 times the volume of column) and the eluting velocity was 6 ml·min^(-1).Conclusion:AB-8 resin could be used to purify TGP successfully, the purity of paeoniflorin was 46.49%.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第7期610-612,共3页
Lishizhen Medicine and Materia Medica Research
