摘要
目的:建立裸茎金腰中2种黄酮醇反相高效液相色谱测定方法。方法:色谱柱为 Kromasil ODS-1柱(5μm,4.6 mm×250 mm),Kromasil ODS-1保护柱(5 μm,4.6 mm×10 mm)。流动相为甲醇-水(60:40),检测波长350 nm,柱温25℃;流速1.0 mL·min^(-1);进样量:10μL。结果:黄酮醇1(5,4′-dihydroxy-3,6,3′-trimethoxy-flavone-7-O-β-D-gluco-side)浓度在84.5-507.2μg·mL^(-1)范围内与峰面积有良好的线性;黄酮醇2(veronicafolin)浓度在36-216μg·mL^(-1)范围内与峰面积有良好的线性。回收率分别为99.77%及98.86%,精密度试验 RSD 分别为1.2%及1.5%(n=5)。结论:该方法灵敏、重复性好、简单可行、结果可靠,可准确定量,从而为藏药裸茎金腰的质量控制提供依据,并为科学评价其药材质量提供有效手段。
Objective:To develop an RP-HPLC method for the determination of two flavonols [5,4′-dihydroxy- 3,6,3′-trimethoxy-flavone-7-O-β-D-glucoside (flavonol 1) and veronieafolin (flavonol 2)]from Tibetan medicine Chrysosplenium nudicaule Bunge.Methods:Using a Waters 600E HPLC series [Kromasil ODS-1 column (5 μm,4.6mm×250 mm),Kromasil ODS-1 protect column(5μm,4.6 mm×10mm)] in an oven at 25℃, with a solvent system consisting of MeOH-H_2O(60:40)and the detective wavelength is 350 nm,the flow rate was 1mL·min^(-1).Results:There were better linear relationships in the ranges of 84.5-507.2μg·mL^(-1) for flavonol 1 and 36-216 μg·mL^(-1) for flavonol 2,respectively.The average recoveries (n=5) for flavonol 1 and flavonol 2 were 99.77% (RSD=1.2%),98.86% (RSD=1.5%) respectively.Conclusion:The method is reproducible,depend- able,simple and useful in quality evaluation of the crude medicine of Chrysosplenium nudicaule Bunge.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第4期418-420,共3页
Chinese Journal of Pharmaceutical Analysis
