摘要
对引自Ｂｏｔｓｗａｎａ西瓜种质资源的抗病性鉴定结果表明，在５ｘ１０￣５孢子／ｍｌ及浸根５ｍｉｎ的压力下，ＭＫ＿２、Ｍ＿２对２号小种表现免疫，ＭＫ＿２、Ｗ＿３及ＬｏｖｒｉｎｘＷ＿３对０号小种表现免疫；田间调查表明，对炭疽病的抗性普遍较强（Ｔ＿１和ＭＫ除外），对病毒病的抗性和耐性普遍强于对照，尤其ＭＫ＿２对枯萎病和炭疽病表现免疫，对病毒病表现高抗；大部分资源的叶片和根系中拥有比对照丰富的分子量较大的ＰＯＤ同工酶，种子球蛋白组分比对照丰富。
The disease resistance of some watermelon gerplasm introduced from the Botswana(the origin of watermelon),Africa ,was studied ,The true leaf and root POD isoenzyme and the seed globulin were analysed by means of electrophoresis. The results of artificial inoculation identification at its seedling stage showed that the MK_2 ,M_2 were not infected by race 2 and the MK_2,M_3,lovrin xW_3 were not infected by race 0 under 5 x 10￣5 spores/ml inoculation presure;The field investigation sbowed that the resis- tance of these germplasm(T_1 and MK were exceped)to anthracnose is much higher than that of CK (commercial cultivar).The rate of virus disease in field showed that these germplasm have much higher resistance and tolerance to virus disease。 The MK_2,which is immune to fusarium wilt and anthracnose and is much higher resistant to virus disease,is hardly to find in citrullus lanatus. The utilization of these germplasm will bring about a great abvance in disease resistance breeding of watermelon. The analysis of POD isoenzyme zymogram showed that the true leaves and roots of the African germplasm have several additional isoenzyme bands. The existing of these bands is probably relative to the disease resistance and other special property of these germplasm. The SDS-PAGE atlas of seed globulin showed that the seeds of these germlasm have richer globulin components. According to POD isoenzyme zymogran and SDS-PAGE atlas,we concluded that these African germplasm have rich genetic materials.The introduction of these germplasm will enrich extremely watermelon germplasm of our country。
出处
《西北农业学报》
CSCD
1994年第2期87-91,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金
关键词
西瓜
种质资源
抗病性
POD同工酶
Watermelon
Germplasm
Disease resistance
POD isoenzyme,Seed globulin
