摘要
应用垂直板不连续系统的聚丙烯酰胺凝胶电泳（ＰＡＧＥ）法，分析了黄瓜农大秋棚二号（Ｆ１）及其双亲发芽种子子叶苹果酸脱氢酶（ＭＤＨ）同工酶酶谱。发现了２个具有多态性的同工酶位点ＭＤＨ—２和ＭＤＨ—４。并以这两个位点为标记检测Ｆ１代（出现“杂种二聚体”）的纯度。对该一代杂种Ｆ２代及ＢＣ１、ＢＣ２代进行的遗传分析表明ＭＤＨ－４位点受一对共显性等位基因（ＭＤＨ－４Ｆ、ＭＤＨ－４Ｓ）遗传控制，ＭＤＨ—２位点存在－隐性虚等位基因（ＭＤＨ—２ｎ），这两个位点均符合孟德尔单基因分离规律，证实了黄瓜杂种一代农大秋棚二号及其双亲间苹果酸脱氢酶同工酶酶谱差异的遗传基础。
Crude extracts from cotyledons of germinating cucumber seeds were separated by electrophoresis on polyacrylamide gel and stained for Malate dehydrogenase (MDH). Polymorphisms were observed at two loci (MDH-4、MDH-2). The cultivar purity of F1 hybrids Nongda qiupeng No.2 were tested by utilizing MDH-4 and MDH-2loci as markers. This method offers a practical and rapid means of estimating the sib frequencies in commercial seed stocks. The analysis of F2 and backcosses progeny revealed that MDH-4 variants are controlled by codominant alleles (MDH-4F. MDH-4s) at one locus and a recessive null allele exists at MDH-2 locus. These two loci demonstrated simple Mendelian inheritance.
出处
《园艺学报》
CAS
CSCD
北大核心
1995年第3期251-255,共5页
Acta Horticulturae Sinica
基金
国家"八五"科技攻关资助
关键词
黄瓜
同功酶
电泳
纯度检测
杂种一代
Cucumber (Cucumis Sativus L.), Isozyme
Electrophoresis
Purity testing
