摘要
目的 :建立测定犬体内左氧氟沙星血药浓度的反相高效液相色谱法 ,并测定其犬体内药代动力学。方法 :以环丙沙星为内标 ,采用 10 %高氯酸为沉淀剂处理犬血浆样品 ,色谱柱为DIKMADiamon silTM C18,5m ,15 0mm× 4 .6mm ,流动相为甲醇 PBS 三乙胺 异丙醇 (33 6 7 0 .14 0 .4 ,用磷酸调pH为 3.0 ) ,流速 1.2ml·min-1,柱温 2 0℃ ,荧光检测(λex=2 95nm ,λem=4 40nm)。结果 :血药浓度线性范围 0 .2 5～ 5 0mg·L-1(r =0 .9998) ,最低检测限浓度为 0 .0 2mg·L-1(S N >3) ,绝对回收率为 83.0 %～85 .6 % (n =5 ) ,方法回收率为 92 .10 %～ 10 6 .77%(n =5 ) ,日内和日间RSD分别为 1.3%～ 4 .0 %和1.8%～ 8.2 % (n =5 )。结论 :本方法简便、灵敏、精密度高 ,重现性好 。
AIM: To establish a HPLC method for the determination of levofloxacin in dog plasma and its pharmacokinetics in dogs. METHODS: The plasma protein was precipitated by the addition of 10% perchloric acid. The resulting supernatant liquid was directly injected after centrifuge and dilution. The chromatography conditions were: DIKMA column (Diamonsil TM C 18 5 μm, 150 mm× 4.6 mm),methanol-PBS-triethylamine-isopropanol (33670,14 0.4,adjusted pH to 3.0 with H 3PO 4) as mobile phase with a flow rate of 1.2 ml·min -1. It was detected by fluorescence detector, and the detection wavelength was λ ex= 295 nm and λ em= 440 nm. Ciprofloxacin was the internal standard. RESULTS: The linear range of calibration curve was within drug plasma concentrations of 0.25- 50 mg·L -1 (r= 0.9998), the detection limit was 0.02 mg·L -1(S/N>3), the extraction recovery was 83.0%- 85.6% (n=5), the method recovery was 92.10%- 106.77%(n=5),the within-day RSD and between-day RSD were 1.3%- 4.0% and 1.8%- 8.2% (n=5), respectively. CONCLUSION: The method possesses merits of simplicity, sensitivity, good accuracy, high precision, and fine reproducibility, which is suitable for pharmacokinetic and bioavailability studies of levofloxacin in dogs.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第6期669-672,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
江苏省药代动力学重点实验室资助项目 (№BM2 0 0 12 0 1)
