摘要
目的研究托瑞米芬(TOR)联合吉西他滨(GEM)对人肺癌株A549的影响,为肺癌的临床治疗提供新的方向。方法用MTT比色法检测各组药物对细胞生长的抑制作用,用HE染色观察用药后细胞形态,用细胞凋亡试剂盒检测细胞凋亡率。结果托瑞米芬能直接抑制肺腺癌细胞A549的生长,低剂量托瑞米芬(5、10μmol/L)与吉西他滨联用后的抑制率较单用吉西他滨明显提高,并且随药物浓度增高而增高。联合用药组较单用化疗药物组凋亡率提高,而单用托瑞米芬对细胞的凋亡率与对照组比无明显变化。结论托瑞米芬可抑制肺腺癌细胞A549的生长,但不能直接促进细胞凋亡;托瑞米芬与吉西他滨有明显的协同作用。
Objective To study synergistic effect of toremifene combined with gemcitabine on human lung cancer line A549,and to provide a new clinical approach for lung cancer.Methods Cell proliferation was evaluated by MTT assay.Morphological change of cell apoptosis was observed by HE stains.Apoptosis rate was detect by using an in situ cell death detection kit.Results Toremifene could inhibit the growth of A549 cells.The inhibitory rates of the low-dose toremifene(5,10 μmol/L)combined with gemcitabine were significantly higher than that of gemcitabine alone,and increased with increasing agent concentration.Morphologic changes induced by drugs were observed through HE and Tunel stains.Apoptosis rate induced by the combination of toremifene and gemcitabine was higher than that of anti-tumor drug alone,but apoptosis rate induced by toremifene alone had no significant difference compaired with that of control group.Conclusion Toremifene could inhibit the growth of human lung adenocarcinoma cell line A549.And toremifene itself could not accelerate apoptosis of cancer cell directly.Toremifene combined with gemcitabine showed significant synergistic anti-tumor effects on cell line A549.
出处
《中国病毒病杂志》
CAS
2008年第3期188-191,共4页
Chinese Journal of Viral Diseases
关键词
托瑞米芬
吉西他滨
化疗
肺腺癌细胞
协同作用
Toremifene
Gemcitabine
Chemotherapy
Lung adenocarcinoma cell
Synergistic effect
