摘要
目的研究川芎嗪对氧化低密度脂蛋白(ox-LDL)诱导的体外动脉粥样硬化(AS)样损伤内皮细胞抗凋亡蛋白Bcl-2及促凋亡蛋白Bax表达的影响,为川芎嗪临床应用提供新的理论及实验依据。方法 ox-LDL(50 mg/L,12 h)诱导体外培养的人冠状动脉内皮细胞,加入川芎嗪(1和10μmol/L),Western blot检测川芎嗪对Bcl-2及Bax蛋白表达的影响。结果 ox-LDL可抑制AS样损伤细胞模型Bcl-2蛋白的表达(与正常对照组比较,ox-LDL模型组Bcl-2与β-actin的相对光密度比值显著降低:0.33±0.08 vs.0.58±0.09,P<0.01),增加Bax蛋白的表达(ox-LDL模型组vs.正常对照组:0.67±0.10 vs.0.21±0.05,P<0.01);川芎嗪则可有效逆转上述病理改变[川芎嗪(1和10μmol/L)显著增加Bcl-2蛋白的表达:0.55±0.06,0.61±0.10,P<0.01;显著降低Bax蛋白表达:0.15±0.09,0.18±0.06,P<0.01]。结论川芎嗪靶向内皮细胞,抑制ox-LDL诱导的AS早期内皮细胞的凋亡反应,从而有效干预AS早期泡沫细胞形成的始动环节,阻断AS向中晚期进展。
Objective To investigate the effects of ligustrazine on expressions of Bcl-2 and Bax in ox-LDL-induced atherosclerotic cell model through improving endothelial functions,and providing a new theoretical and experimental basis for clinical application.Methods In in vitro experiments,human coronary aortic endothelial cells(HCAEC)were treated by ox-LDL(50 mg/L,12 h) to mimic in vivo atherosclerosis formation.Further investigation had been carried out through examining the effects of ligustrazine(1and10 μmol/L) on the protein expression of Bcl-2 and Bax.Results Ligustrazine can increase the protein expression of Bcl-2(0.55±0.06,0.61±0.10,P<0.01),inhibit the protein expression of Bax(0.15±0.09,0.18±0.06,P<0.01) at the concentration of 1 and 10 μmol/L.Conclusions It is suggested that ligustrazine can intervene endothelial cells apoptosis and the intiation step of atherosclerosis.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第23期6898-6901,共4页
Chinese Journal of Clinicians(Electronic Edition)
关键词
动脉粥样硬化
内皮细胞
川芎嗪
细胞凋亡
Atherosclerosis
Endothelial cells
TETRAMETHYLPYRAZINE
Apoptosis
