摘要
目的:研究主要理化因子对西洋参细胞悬浮培养的影响。方法:利用组织培养技术结合高效液相色谱法和紫外分光光度法,考察接种量、培养基种类、基质pH和光照条件对西洋参悬浮细胞生长,人参皂苷Re,Rb1以及西洋参多糖含量的影响。结果:当接种量为25g·L-1时,西洋参细胞的干重增殖倍数显著增加;通过考察MS,SH,B53种培养基对西洋参细胞的影响,结果表明MS培养基最有利于西洋参细胞生长,B5培养基最有利于人参皂苷和西洋参多糖的合成。3种培养基中西洋参细胞多糖含量均高于栽培西洋参;pH变化对西洋参细胞生长影响不大,pH6.0时,最有利于人参皂苷Re和西洋参多糖的合成;光照培养显著促进西洋参细胞次生代谢物的合成,但对多糖合成没有太大影响。结论:接种量、培养基种类、基质pH和光照条件对西洋参悬浮细胞生长,人参皂苷Re,Rb1以及西洋参多糖合成有显著影响。
Objective:To study the effects of inoculum,various media,pH value of medium and illumination conditions on the growth of Panax quinquefolium suspension cells and the synthesis of ginsenosides Re,Rb1 and polysaccharides.Method:The suspension cells were obtained through tissue culture by manipulation of inoculum,various media,pH value,and illumination conditions.The contents of ginsenosides Re and Rb1 were determined by HPLC,while the contents of polysaccharide were determined by ultraviolet spectrophotometry...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第4期375-378,共4页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划项目(2006BAI06A16-02)
关键词
西洋参
细胞悬浮培养
接种量
培养基
人参皂苷
西洋参多糖
Panax quinquefolium
cell suspension culture
inoculum
medium
ginsenoside
polysaccharide
