摘要
目的 探讨miR-146a rs2910164位点单核苷酸多态性(single nucleotide polymerphism,SNP)与类风湿关节炎(rheumatoid arthritis,RA)病情活动及临床表现的相关性.方法 以高分辨率熔解(high-resolution melting,HRM)法对126例RA患者和102名正常对照进行miR-146a rs2910164位点的SNP分型,比较各基因型及等位基因频率在正常对照和RA患者中的差异,并分析其与RA患者病情活动性及临床表现之间的关系.结果 RA患者的miR-146a rs2910164位点G等位基因频率(30.6%)低于正常对照(32.8%),而C等位基因频率(69.4%)高于正常对照(67.2%);RA患者中GG基因型的比例(12.7%)低于正常对照(14.7%),而CC基因型的比例(51.6%)高于正常对照(49.O%),但差异均无统计学意义.具有GC基因型的RA患者出现关节外表现的比例显著增高(P<0.01),但是并未发现miR-146a rs2910164位点的不同基因型之间的DAS28评分的差异有统计学意义(P>0.01).结论 miR-146a rs2910164位点SNP非RA发病的独立风险因素,并且与RA病情活动性无明显相关性,但miR-146a rs2910164位点的GG基因型与RA特定的临床表现有关,提示miR-146a可能在RA发病中具有一定的作用.
Objective To assess the association of single nucleotide polymorphisms(SNPs)of microRNA-146a(miR-146a)with clinical features of rheumatoid arthritis(RA).Methods In 126 patients with RA and 102 matched healthy controls,SNPs of miR-146a rs2910164 locus were determined with a high-resolution melting method.The association of such polymorphisms with disease activity score in 28 joints(DAS28)and clinical features of RA was assessed.Results The distribution of SNPs of miR-146a rs2910164 among RA patients did not differ from that of the control group.No significant association was found between miR-146a rs2910164 polymorphism with DAS28.However,RA patients with a GG genotype had a higher chance to develop extraarticular manifestations(P<0.01).Conclusion Polymorphisms of miR-146a rs2910164 locus is not an independent risk factor for RA,though its GG genotype may be associated with extraarticular manifestations of RA.
作者
胡秋侠
李博
佘若男
吴系美
谭锦辉
胡建云
唐群芳
Hu Qiuxia;Li Bo;She Ruonan;Wu Ximei;Tan Jinhui;Hu Jianyun;Tang Qunfang(Department of Rheumatology,People’s Hospital of Longhua District of Shenzhen,Shenzhen,Guangdong 518109,China)
出处
《中华医学遗传学杂志》
CAS
CSCD
2019年第5期505-507,共3页
Chinese Journal of Medical Genetics
基金
广东省医学科研基金立项课题(A2013611)
深圳市卫生计生系统科研项目(201302182,201501051,201601060)
深圳市龙华区科技创新资金项目(20170919A1030357).
作者简介
通信作者:李博,Email:friendlybo@163.com
