摘要
目的基于指纹图谱、聚类分析与主成分分析方法为不同产地荆芥药材质量控制提供参考。方法利用高效液相色谱法对荆芥药材进行质量分析,所获数据用中药色谱指纹图谱相似度评价系统构建荆芥药材的指纹图谱,并应用SPSS 23.0统计软件进行聚类分析和主成分分析,综合评价不同产地荆芥药材的质量。色谱柱为SinoChrom ODS-BP(4.6mm×250mm,5μm);流动相为乙腈-0.05%磷酸水溶液,梯度洗脱;检测波长:283nm;流速:1.0mL/min;柱温:30℃;进样量:20μL。结果荆芥药材高效液相色谱指纹图谱28个峰确定为共有峰,除河南鹿邑、四川自贡的样品相似度低于0.9外,其他的21批荆芥药材的相似度为0.930~0.982。聚类分析结果表明,荆芥药材可被分为亳州荆芥与四川、河南、河北等其他产地荆芥两大类。主成分分析结果表明,前6个主成分的累计贡献率达到88.247%,选择这6个因子可对不同产地荆芥药材进行综合分析。结论根据最终的综合得分结果可知,安徽、河北产荆芥药材质量相对较好。
Objective To provide a reference for quality control of fineleaf Schizonepeta herb from different producing areas based on fingerprint,cluster analysis,and principal component analysis.Methods Highperformance liquid chromatography(HPLC)was used to analyze the quality of fineleaf Schizonepeta herb,and the data obtained were analyzed by the chromatographic fingerprint similarity evaluation system of traditional Chinese medicine to establish the fingerprint of fineleaf Schizonepeta herb.SPSS 23.0 was used for cluster analysis and principal component analysis,and all data were used to evaluate the quality of fineleaf Schizonepeta herb from different producing areas.HPLC was performed on a SinoChrom ODS-BP column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.05%aqueous phosphoric acid solution for gradient elution,at a detection wavelength of 283 nm,a flow rate of 1.0 mL/min,a column temperature of 30℃,and an injection volume of 20μL.Results A total of 28 peaks of the HPLC fingerprint of fineleaf Schizonepeta herb were determined to be common peaks,and apart from the samples from Henan Luyi and Sichuan Zigong with a similarity of below 0.9,all the other 21 batches had a similarity of 0.930-0.982.The cluster analysis showed that the medicinal material of fineleaf Schizonepeta herb was classified into Bozhou fineleaf Schizonepeta herb and fineleaf Schizonepeta herb from other producing areas including Sichuan,Henan,and Hebei.The principal component analysis showed that the top six principal components had a cumulative contribution rate of 88.247%and could be used for the comprehensive analysis of fineleaf Schizonepeta herb from different producing areas.Conclusion The comprehensive analysis shows that fineleaf Schizonepeta herb from Anhui and Hebei has relatively good quality.
作者
廖延武
吴德玲
许凤清
张伟
黄琪
王桐生
LIAO Yan-wu;WU De-ling;XU Feng-qing;ZHANG Wei;HUANG Qi;WANG Tong-sheng(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Anhui Provincial Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces,Anhui Hefei 230012,China;Department of Pharmacology,Anhui University of Chinese Medicine,Hefei Anhui 230012,China)
出处
《安徽中医药大学学报》
2020年第1期78-83,共6页
Journal of Anhui University of Chinese Medicine
基金
国家重点研发计划(2018YFC1707004)
国家中药标准化项目(ZYBZH-C-AH-02)
安徽省高校科研创新平台——中药饮片产地加工与炮制一体化关键技术研究创新团队项目(皖教秘科〔2015〕49号).
关键词
荆芥
高效液相
指纹图谱
聚类分析
主成分分析
Fineleaf Schizonepeta herb
High-performance liquid chromatography
Fingerprint
Cluster analysis
Principal component analysis
作者简介
廖延武(1994-),男,硕士研究生;通信作者:王桐生(1972-),男,教授,硕士研究生导师,wtsyl@163.com
