摘要
目的研究瑶药童子益母草与益母草多指标成分含量差异,并结合含量对品质进行评价。方法采用高效液相色谱法测定瑶药童子益母草与益母草中绿原酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷5种化学成分含量,利用SPSS 25.0软件进行成对样品t检验及单因素方差分析推断各样品间含量差异,主成分分析评价药材品质。结果童子益母草与益母草成对样品t检验结果表明,绿原酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷5种化学成分中芦丁含量呈显著性差异,其余成分无统计学差异;单因素方差分析结果表明,不同产地童子益母草和益母草药材中5种化学成分均存在极显著性差异(P<0.01);主成分分析结果显示,益母草得分较高的产地为南宁和玉林,上思县得分最低。结论瑶药童子益母草与益母草含量存在显著差异,南宁和玉林的药材质量较佳,为2种益母草及不同产地益母草间含量的比较提供了数据支持。
Objective To study the content difference of multi-index components between Yao medicine Young Leonurus heterophyllus(YLH) and Leonurus japonicus, and evaluate the quality with the content. Methods A high performance liquid chromatography(HPLC) method was established for the simultaneous content determination of five chemical constituents of chlorogenic acid, leonurine hydrochloride, rutin, hyperin and isoquercitrin in Yao medicine YLH and Leonurus japonicus. SPSS 25.0 statistical software was used to conduct paired samples t-test and one-way ANOVA to infer the content differences among the samples. Principal component analysis(PCA) was used to evaluate the quality of medicinal materials. Results T-test results of paired samples of Yao medicine YLH and Leonurus japonicus showed that there was significant difference in rutin content among the five chemical constituents, with no significant difference in other constituents. The results of one-way ANOVA analysis showed that there were significant differences in five chemical constituents of Yao medicine YLH and Leonurus japonicus from different habitats(P < 0.01). The results of principal component analysis showed that the higher scores of Leonurus japonicus from different habitats were from Nanning and Yulin, with the lowest in Shangsi County. Conclusion There is a significant difference between the content of Yao medicine YLH and Leonurus japonicus. The quality of medicinal materials from Nanning and Yulin is better. The results provide data support for the comparison of the content between two kinds of Leonurus heterophyllus and Leonurus japonicus from different habitats.
作者
杨帆
吴梦丽
何秋梅
高慧
李嫁炎
谢澳
黎芳婷
谢阳姣
YANG Fan;WU Meng-li;HE Qiu-mei;GAO Hui;LI Jia-yan;XIE Ao;LI Fang-ting;XIE Yang-jiao(College of Yao Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China;Scientific Experimental Center of Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第3期757-762,共6页
Chinese Traditional and Herbal Drugs
基金
广西研究生教育创新计划资助项目(YCSW2018176)
广西壮瑶医药与医养结合人才小高地(厅发[2017] 44号).
关键词
瑶药童子益母草
益母草
绿原酸
盐酸益母草碱
芦丁
金丝桃苷
异槲皮苷
主成分分析
Yao medicine Young Leonurus heterophyllus Sweet
Leonurus japonicus Houtt.
chlorogenic acid
leonurus hydrochloride
rutin
hyperoside
isoquercetin
principal component analysis
作者简介
杨帆,女,硕士研究生,研究方向为中药、民族药资源开发与利用研究。Tel:15677166963,E-mail:15677166963@163.com;通信作者:谢阳姣,女,博士,研究员,研究方向为中药、民族药资源开发与利用研究。Tel:18172384560,E-mail:xieyangjiao@163.com
