摘要
目的:利用公共数据库和临床募集患者两个独立的数据源,探讨儿童急性骨髓性白血病(pediatric acute myeloid leukemia, PAML)差异基因图谱并进行临床验证。方法:利用GEO数据库中的基因芯片数据,对比PAML患者和正常儿童基因数据,寻找差异化基因;将潜在的差异基因进行富集分析。利用天津市儿童医院随机募集PAML患者,针对重点的差异基因进行临床检验。结果:数据库分析中,与正常儿童组相比,PAML患者表现出501个差异基因,包括417个上调基因和84个下调基因。其中,DEFA1B和LTF基因差异最为明显。501个差异表达基因主要集中在immunoglobulin complex等生物途径及Hedgehog等分子通路中。临床患者外周血检验中,DEFA1B在对照组中浓度为(41.68±8.66)ng/mL,PAML组中浓度为(87.12±17.53)ng/mL,差异具有统计学意义(P<0.05)。LTF在对照组中浓度为(23.74±8.03)ng/mL,PAML组中浓度为(61.23±18.65)ng/mL,差异具有统计学意义(P<0.05)。DEFA1B和LTF在儿童慢性髓性白血病(pediatric chronic myeloid leukemia, PCML)组患者中未表现出明显的变化(P>0.05)。结论:PAML患者存在差异性表达基因,Hedgehog等分子通路异常表达,其中DEFA1B和LTF可以作为潜在的临床标志物进行研究。
Objective: To explore the differential gene map of pediatric acute myeloid leukemia(PAML) using two independent data sources(public databases as well as clinical recruitment of patients). Methods: Based on the gene chip data in GEO database, this study compared the differentially expressed genes between PAML patients and normal children, and performed an enrichment analysis of potential differential genes. Based on the randomly recruited PAML patients from Tianjin Children’s Hospital, this work conducted a clinical test on the potential differential genes. Results: Compared with the normal child group, PAML patients showed 501 differential genes, including 417 up-regulated genes and 84 down-regulated genes. Among them, DEFA1B and LTF genes were the most significant. The 501 differentially expressed genes were mainly enriched in biological processes such as immunoglobulin complex and molecular pathways such as Hedgehog. In the peripheral blood test of clinical patients, the concentration of DEFA1B in the control group was(41.68±8.66) ng/mL, and the concentration in the PAML group was(87.12±17.53) ng/mL, as the difference was statistically significant(P<0.05). The concentration of LTF in the control group was(23.74±8.03) ng/mL, and the concentration in the PAML group was(61.23±18.65) ng/mL, as the difference was statistically significant(P<0.05). The concentration of DEFA1B and LTF in children with chronic myeloid leukemia(PCML) group did not show a significant change(P>0.05). Conclusion: PAML patients have differentially expressed genes, and molecular pathways such as Hedgehog are abnormally expressed. Among them, DEFA1B and LTF can be studied as potential clinical markers.
作者
崔晓鸣
高伟
何娟娟
张博
CUI Xiaoming;GAO Wei;HE Juanjuan;ZHANG Bo(Tianjin Children's Hospital,Department of Laboratory,Tianjin 300074,CHN;Department of Immunology/Tianjin Key Laboratory of Cellular and Molecular Immunology,School of Basic Medical Sciences,Tianjin 300070,CHN)
出处
《河南大学学报（医学版）》
CAS
2023年第1期23-27,共5页
Journal of Henan University:Medical Science
基金
国家自然科学基金面上项目(82070687)
作者简介
崔晓鸣(1983-),女,学士,主管技师。研究方向:临床检验;通信作者:张博,E-mail:bozhang@tmu.edu.cn
