摘要
建立茶叶中多菌灵残留的高效液相色谱-串联四极杆质谱联用测定方法。该方法中样品经0.2 mol/L盐酸甲醇溶液(1∶1,υ/υ)超声提取,MCX固相萃取柱净化,以ZORBAX Eclipse XDB-C18色谱柱(3.0 mm×50 mm,1.8μm)分离,流动相为0.1%甲酸水和乙腈(梯度洗脱),电喷雾正离子MRM模式检测。该方法的检出限为1.0μg/kg,线性范围0.5μg/L～3 000μg/L,加标回收率为85.0%～96.0%,相对标准偏差为3.71%。
A novel method for the determination of carbendazim in tea by LC-MS.In the method,sample was extracted with methanol-0.2 mol/L HCl（1：1,υ/υ）,purified by MCX solid-phase extraction cartridge,Chromatographic separation was carried out on a ZORBAX Eclipse XDB-C18 column（3.0 mm×50 mm,1.8 μm）,the mobile phase was acetonitrile-0.1 % formic acid water solution with gradient elution.The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring.The limit of detection was 1.0 μg/kg,the linear plots were obtained between 0.5 μg/L and 3 000 g/L.Overall recoveries were between 85.0 % and 96.0 %,and RSD value was 3.71 %.
出处
《食品研究与开发》
CAS
北大核心
2012年第5期111-114,共4页
Food Research and Development
作者简介
田艳铃（1966-），女（汉），高级工程师，学士，从事食品检验的实验工作
通信作者：王浩（1969-），男，工程师，硕士研究生，从事食品安全与营养检验的研究工作
