摘要
克罗恩病(Crohn’s disease,CD)是一种特殊的肠道免疫性疾病,其发病机制一直是医学界的热点与难点,临床上无法得到治愈,因此选择合适的方法建立与临床吻合度较高的CD动物模型对于发病机制的研究至关重要。本文通过整理国内外研究文献,总结现有CD动物模型的特点以及中西医临床诊断标准,并对CD动物模型与临床的吻合度进行赋值。结果显示复制CD动物模型的方法较多,其中以病证结合动物模型是较为理想的选择。但是对于病证结合的CD模型应用少之又少,大多数模型以西医致病因素为主导缺少中医致病的干预,因此可以考虑在西医致病因素的基础上施加以湿热蕴结证、寒湿困脾证、气滞血瘀证、肝郁脾虚证和脾胃虚寒证等证型的干预因素并加以区分,获得具有中医证候临床表现的病证结合CD动物模型。此外模型成功标准也需进一步完善,建议增加动物饮食量、精神状态、体重、毛发以及粪便等表观指标的量化评分标准。完善造模方法和模型评判标准是建立高度模拟临床CD复杂病理表现的CD动物模型的关键,同时为深入研究CD提供更加完善的实验研究载体。
Crohn's disease(CD)is a special intestinal immune disease,and its pathogenesis has been a hotspot and difficult issue in the medical field,which cannot be cured clinically.In this paper,we summarized the characteristics of existing CD animal models and clinical diagnostic criteria of Chinese and Western medicine by compiling domestic and international research literature,and assigned values to the clinical fit of CD animal models.The results showed that there are many methods to replicate CD animal models,among which the pathology-evidence combination animal model is the more ideal choice.Most of the models were dominated by Western medicine pathogenic factors without the intervention of Chinese medicine pathogenic factors.Therefore,we can consider applying the intervention factors of Damp-Heat,Cold-Damp Trapped Spleen,Qi Stagnation and Blood Stasis,Liver Stagnation and Spleen Deficiency,and Spleen and Stomach Deficiency Cold on the basis of Western medicine pathogenic factors and differentiate them to obtain the pathogenic CD models with clinical manifestations of Chinese medicine symptoms.In addition,the success criteria of the model need to be further improved,and it is recommended to increase the quantitative scoring criteria of the animal's diet,mental status,weight,hair and feces and other apparent indicators.Improving the modeling methods and model criteria is the key to establishing an animal model of CD that highly simulates the complex pathological manifestations of clinical CD,and provides a more complete experimental research vehicle for in-depth studies of CD.
作者
赵明明
苗明三
Zhao Mingming;Miao Mingsan(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第11期3751-3757,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
河南省科学技术厅河南省重大公益专项(201300310100):豫产道地中药材资源综合利用研究,负责人:苗明三
关键词
克罗恩病
中西医
病症特点
诊断标准
动物模型
Crohn's disease
Chinese and Western medicine
Disease characteristics
Diagnostic criteria
Animal models
作者简介
通讯作者:苗明三,教授,主要研究方向:中药药理学
