摘要
目的对我国北京地区肉类产品中沙门氏菌进行分离鉴定,基于全基因组测序结果,分析其耐药基因分布情况及亲缘关系。方法以38株沙门氏菌为研究对象,对不同来源的沙门氏菌进行全基因组分析,然后通过测定菌株对15种抗生素的最小抑菌浓度,结合全基因组测序结果分析不同来源的沙门氏菌的耐药特性及多重耐药状况。结果对38株沙门氏菌进行15种抗生素耐药性检测,其中34.2%为多重耐药株,68.4%的沙门氏菌对喹诺酮萘啶酸耐药,42.1%分离株对氨苄西林耐药。基于全基因组测序结果,对38株沙门氏菌进行分型为12种型别,其中88.2%的肠炎沙门氏菌与标准菌株ATCC9184亲缘关系较近。结论全基因组耐药基因和毒力基因与耐药表型有一定的关联,为监控北京地区的沙门氏菌耐药基因和毒力基因的传播方式提供理论基础。
Objective To isolate and identify Salmonella in meat products in Beijing,China.Based on the results of genome-wide sequencing,the distribution of drug resistance genes and genetic relationship were analyzed.Methods We used 38 strains of Salmonella to study genome-wide analysis of Salmonella from different sources.Then,by measuring the minimum inhibitory concentration of the strains against 15 antibiotics,combined with the results of genome-wide sequencing,the drug resistance characteristics and multidrug resistance of Salmonella from different sources were analyzed.Results Thirty eight Salmonella strains were tested for resistance to 15 antibiotic,of which 34.2%were multidrug resistant strains,68.4%of which were resistant to quinolone nalidixic acid,and 42.1%of isolates were resistant to ampicillin.Based on the results of genome-wide sequencing,38 strains of Salmonella were classified into 12 types,88.2%of which were closely related to the standard strain ATCC9184.Conclusion The genome-wide resistance genes and virulence genes are related to the drug resistance phenotype,which provides a theoretical basis for monitoring the transmission of Salmonella resistance genes and virulence genes in Beijing.
作者
畅晓晖
张捷
亓合媛
石嵩
杨向莹
杨磊
赵琢
李小林
史文聿
孙清岚
马俊才
陈广全
CHANG Xiao-Hui;ZHANG Jie;QI He-Yuan;SHI Song;YANG Xiang-Ying;YANG Lei;ZHAO Zhuo;LI Xiao-Lin;SHI Wen-Yu;SUN Qing-Lan;MA Jun-Cai;CHEN Guang-Quan(Technical center of Beijing Customs District People’s Republic of China,Beijing 100026,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Tianjin Customs Industrial Product Safety Technology Center,Tianjin 300000,China)
出处
《食品安全质量检测学报》
CAS
2020年第3期783-791,共9页
Journal of Food Safety and Quality
基金
国家重点研发计划重点专项(2018YFC1603800)
海关总署科技计划项目(2017IK157).
关键词
沙门氏菌
全基因组测序
耐药基因
耐药表型
Salmonella
genome-wide sequencing
drug resistance gene
drug resistance phenotype
作者简介
畅晓晖,硕士,工程师,主要研究方向食品安全检测。E-mail:changxiaohui82@163.com;通讯作者:赵琢,博士,研究员,主要研究方向为食品安全检测。Email:zhaozhuo@customs.gov.cn
