摘要
目的研究茶园土壤真菌Fusariums sp.CT01-P-5次级代谢产物,以期得到结构新颖的先导化合物。方法采用硅胶柱色谱、ODS反相柱色谱、凝胶柱色谱及高效液相色谱等方法进行分离纯化,通过理化性质及谱学数据分析鉴定化合物结构。结果从茶园土壤真菌Fusariums sp.CT01-P-5发酵液中分离得到15个化合物,分别鉴定为Cyclo(S-Phe-S-Ser)(1)、Cyclo(R-Phe-S-Ser)(2)、Cyclo(4-hydroxyl-S-Pro-S-Phe)(3)、Cyclo(4-hydroxyl-R-Pro-S-Phe)(4)、lateritin(5)、beaurericin(6)、Cyclo(R-Pro-S-Phe)(7)、Cyclo 1-[2-(cyclopentanecarbonyl-3-phenryl-propionyl]-pyrrolidine-2-carboxylic acid(1-carbamoyl-propyl)-amide(8)、Cyclo(Pro-Leu)(9)、Cyclo(Leu-Arg)(10)、Cyclo(Try-Pro)(11)、4-hydroxyphenylacetic acid methyl ester(12)、phenylacetic acid(13)、N-(2-phenylethyl)acetamide(14)和Cyclo(S-Tyr-4-hydroxyl-S-Pro)(15)。结论化合物1、2、8为首次从镰刀霉属真菌次级代谢产物中分离得到,其余化合物均为首次从茶园土壤微生物中分离得到。
Objective To obtain lead compounds by studying the secondary metabolites from the tea garden soil fungus Fusariums sp.CT01-P-5.Methods The compounds were isolated by silica gel,ODS reverse phase column chromatography,Sephadex LH-20 and high performance liquid chromatography(HPLC).The structures of the compounds were identified by physic-chemical properties and spectroscopic data.Results Fifteen compounds were isolated from Fusariums sp.CT01-P-5 secondary metabolites,which were identified as the Cyclo(S-Phe-S-Ser)(1),Cyclo(R-Phe-S-Ser)(2),Cyclo(4-hydroxyl-S-Pro-S-Phe)(3),Cyclo(4-hydroxyl-R-Pro-S-Phe)(4),lateritin(5),beaurericin(6),Cyclo(R-Pro-S-Phe)(7),Cyclo 1-[2-(cyclopentanecarbonyl-3-phenryl-propionyl]-pyrrolidine-2-carboxylic acid(1-carbamoyl-propyl)-amide(8),Cyclo(Pro-Leu)(9),Cyclo(Leu-Arg)(10),Cyclo(Try-Pro)(11),4-hydroxyphenylacetic acid methyl ester(12),phenylacetic acid(13),N-(2-phenylethyl)acetamide(14)and Cyclo(S-Tyr-4-hydroxyl-S-Pro)(15).Conclusion Compounds 1,2,8 were isolated from the Fusariums sp.for the first time.The other compounds were isolated from the tea garden fungus for the first time.
作者
卢轩
冯伟星
孔萌
王海峰
冯宝民
唐川
LU Xuan;FENG Weixing;KONG Meng;WANG Haifeng;FENG Baomin;TANG Chuan(School of Life Science and Technology,Dalian University,Dalian 116622,China;School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2020年第1期1-6,共6页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(21606030
81341133).
关键词
茶园土壤真菌
液体发酵
次级代谢产物
结构鉴定
tea garden soil fungus
liquid fermentation
secondary metabolite
structural identification
作者简介
卢轩(1984-),男(汉族),山东青岛人,副教授,博士,主要从事天然药物化学方面的研究,Tel.18042615621,E-mail:luxuan_232@163.com;通信作者:唐川(1986-),男(汉族),黑龙江佳木斯人,讲师,博士,主要从事天然药物化学方面的研究,Tel.15542469569,E-mail:jiemotc@gmail.com。
