摘要
目的基于红外光谱技术和化学模式识别方法对生甘草及蜜炙甘草进行化学模式的分析和识别。方法先用红外光谱仪测出生甘草及蜜炙甘草样品的红外光谱,分析二者红外光谱差异;利用spss19.0、SIMCA 14.1分析软件进行二者红外光谱数据的相似性分析、聚类分析、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)等化学模式判别分析。结果生甘草及蜜炙甘草红外共有模式光谱具有很大相似性;通过二者的相似性分析、聚类分析和PCA可知,甘草经蜜炙后化学成分和含量发生了某种意义上的变化,但在一定程度上也保留了共有的特征;所建立的OPLS-DA模型很好的反映出了二者的差异性。建立的化学模式识别方法能够真实、可靠的反映出甘草经蜜炙后特征变化,可作为二者分类和鉴别的指标。结论所建立的生甘草及蜜炙甘草的化学模式判别方法可靠,能够清晰有效地判别生甘草和蜜炙甘草,为运用化学模式判别方法对中药及其炮制品的鉴别和品质评价提供参考。
Objective Based on infrared spectroscopy and chemical pattern recognition,the chemical patterns of Radix Glycyrrhizae and Honey grilled Glycyrrhiza uralensis Fisch.were analyzed and recognized.Methods The infrared spectra of the samples of Radix Glycyrrhizae and Honey grilled Glycyrrhiza uralensis Fisch.were measured by infrared spectrometer,and the differences of infrared spectra between them were analyzed by spss19.0 and SIMCA 14.1 software.The similarity analysis,cluster analysis,principal component analysis(PCA)and orthogonal partial least square discriminant analysis(OPLS-DA)were used to analyze the chemical patterns of the infrared spectral data of Radix Glycyrrhizae and Honey grilled Glycyrrhiza uralensis Fisch..Results Through the similarity analysis,cluster analysis and PCA,we can see that the chemical composition and content of Honey grilled Glycyrrhiza uralensis Fisch.have changed in a certain sense,but to some extent,they also retain the common characteristics,and the established OPLS-DA model well reflects the difference between the two.The established chemical pattern recognition method can truly and reliably reflect the characteristics of Radix Glycyrrhizae after honey processing,and can be used as an index for the classification and identification of Honey grilled Glycyrrhiza uralensis Fisch..Conclusion The chemical model discrimination methods of Radix Glycyrrhizae and Honey grilled Glycyrrhiza uralensis Fisch.established in this experiment are reliable and can clearly and effectively distinguish Radix Glycyrrhizae and Honey grilled Glycyrrhiza uralensis Fisch.,which provides a reference for the identification and quality evaluation of traditional Chinese medicine and its processed products.
作者
张育贵
王临艳
张淑娟
辛二旦
李东辉
吴红伟
司昕蕾
牛江涛
边甜甜
李越峰
ZHANG Yu-gui;WANG Lin-yan;ZHANG Shu-juan;XIN Er-dan;LI Dong-hui;WU Hong-wei;SI Xin-lei;NIU Jiang-tao;BIAN Tian-tian;LI Yue-feng(Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;Key Laboratory of Standard and Quality of Chinese Medicine Research of Gansu,Lanzhou Gansu 730000,China;Clinical Examination Center of Gansu Maternal and Child Health Hospital,Lanzhou Gansu 730050,China;Chinese Medicine Processing and Quality,Corrtral Engineering Technology Research Center of Gansu,Affiliated Hospital of Gansu Chrrirersity of Chinese Medicine Lanzhou Gansu 730000,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2020年第10期2401-2405,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金-地区科学基金项目(81960713)
国家自然科学基金(81460611)
甘肃省自然科学基金创新基地和人才计划项目(18JR3RA197)
甘肃省中药质量与标准研究重点实验室开放基金(ZYZL18-008)
关键词
生甘草
蜜炙甘草
红外光谱
聚类分析
相似性分析
主成分分析
正交偏最小二乘法-判别分析
Radix Glycyrrhizae
Honey grilled Glycyrrhiza uralensis Fisch.
infrared spectrum
Cluster analysis
Similarity analysis
Principal component analysis
Orthogonal partial least square discriminant analysis
作者简介
张育贵(1995-),男(汉族),甘肃定西人,甘肃中医药大学在读硕士研究生,主要从事中药及复方加工炮制机理及活性成分研究工作;通讯作者:李越峰(1976-),女(汉族),甘肃庆阳人,甘肃中医药大学教授,博士研究生导师,博士学位,主要从事中药及复方加工炮制机理及活性成分研究工作.
