摘要
目的观察正己烷亚慢性染毒对大鼠血清中神经元特异性烯醇化酶(NSE)、神经丝轻链(NF-L)和神经生长因子(NGF)水平的影响,探讨NSE、NF-L和NGF作为正己烷神经毒性生物标志物的可行性。方法将无特定病原体级SD雄性大鼠随机分为对照组和低、中、高剂量组,每组6只。低、中、高剂量组大鼠分别予剂量为168、675、2 700 mg/kg体质量正己烷原液灌胃染毒,对照组大鼠予等体积0.9%氯化钠溶液灌胃,每日1次,每周染毒5 d,共染毒6周。在染毒第0、2、4、6周,称量大鼠体质量,进行步态评分,检测大鼠血清中NSE、NF-L和NGF水平。结果大鼠体质量、步态评分和血清中NSE、NF-L水平在正己烷染毒剂量和染毒时间的交互效应上均有统计学意义(P<0.01);在正己烷染毒第6周,3个剂量组大鼠体质量均低于对照组(P<0.05),步态评分均高于对照组(P<0.05),且大鼠步态异常呈随着正己烷染毒剂量增加而严重的剂量-效应关系;在正己烷染毒第2、4、6周,3个剂量组大鼠血清NSE和NF-L水平均高于同时间点对照组(P<0.05);且中、高剂量组大鼠血清NF-L水平均呈随染毒时间增加而升高的时间-效应关系(P<0.05)。大鼠血清NGF水平仅在正己烷染毒剂量和染毒时间的主效应上有统计学意义(P<0.05)。其中,高剂量组大鼠血清中NGF水平分别低于对照组和低、中剂量组(P<0.05);染毒第6周大鼠血清中NGF水平分别低于染毒第0、2、4周(P<0.05)。结论血清中NSE和NF-L均可作为正己烷所致周围神经损伤的早期效应生物标志物;血清NGF作为正己烷所致周围神经损伤的早期效应生物标志的可行性仍有待进一步研究。
Objective To observe the effect of n-hexane subchronic exposure on the serum level of neuron specific enolase(NSE), neurofilament light chain protein(NF-L) and nerve growth factor(NGF) in rat, and to explore the feasibility of using NSE, NF-L and NGF as biomarkers of n-hexane neurotoxicity. Methods Specific pathogen free male SD rats were randomly divided into control group and low-, medium-and high-dose exposure groups, with 6 rats in each group. Rats in the low-, medium-and high-dose exposure groups were given n-hexane solution at doses of 168, 675 and 2 700 mg/kg body mass, respectively, while rats in the control group were gavaged with 0.9% sodium chloride solution, once a day, 5 days a week for 6 weeks. At week 0, 2, 4, and 6 of exposure, the body mass of the rats was weighed, the gait scores were performed, and the serum levels of NSE, NF-L, and NGF were detected.Results Body mass, gait score and serum levels of NSE and NF-L in rats were statistically significant in terms of the n-hexane exposure dose and exposure time(P<0.01). At the 6 th week of n-hexane exposure, the body mass of the three dose exposure groups was lower than that of the control group(P<0.05), and the gait score was higher than that of the control group(P<0.05). Moreover, the abnormal gait of the rats showed a dose-effect relationship with the increasing n-hexane poisoning dose. At week 2, 4 and 6, the serum levels of NSE and NF-L in these three dose exposure groups were higher than that in the control group(P<0.05). In addition, the serum level of NF-L in rats in the medium-and high-dose exposure groups increased with the n-hexane exposure time increasing and showed a time-effect relationship(P<0.05). The level of serum NGF in rats was statistically significant in the main effects of n-hexane dose and duration of exposure(P<0.05). The serum NGF level in the high-dose exposure group was lower than that in the control group, the low-dose and medium-dose exposure groups(P<0.05). NGF level in serum of rats at week 6 was lower than that at week 0, 2 and 4(P<0.05). Conclusion Both NSE and NF-L in serum can be used as biomarkers for the early effect of n-hexane on peripheral nerve injury. The feasibility of using serum NGF as a biomarker for the early effect of n-hexane on peripheral nerve injury warrants further investigation.
作者
彭小粤
马争
彭建梅
王治华
陈满连
蔡木蔚
刘伟东
董晓蕾
余日安
PENG Xiaoyue;MA Zheng;PENG Jianmei;WANG Zhihua;CHEN Manlian;CAI Muwei;LIU Weidong;DONG Xiaolei;YU Rian(Department of Occupational and Environmental Health,School of Public Health,Guangdong Pharmaceutical University,Guangzhou 510310,China;不详)
出处
《中国职业医学》
CAS
北大核心
2019年第6期673-677,共5页
China Occupational Medicine
基金
广东省医学科学技术研究项目(B2018157).
作者简介
彭小粤(1994-),女,在读劳动卫生与环境卫生学硕士研究生,主要从事卫生毒理学研究;通讯作者:余日安,教授,硕士研究生导师,E-mail:yuriantj@163.com;通讯作者:马争,副主任医师,E-mail:zhma2001@163.com
