摘要
[目的]探讨抗核抗体(ANA)和抗可提取性核抗原(ENA)多肽谱检测在自身免疫病(AID)诊断中的应用价值.[方法]检测381例各种AID患者和100例健康人群的ANA和ENA水平并进行分析.[结果]ANA在系统性红斑狼疮(SLE)患者中阳性率最高,为95.5%,抗SSA、抗SSB、抗Sm、抗dsDNA、抗核小体在SLE中的检出率分别为84.8%,76.2%,36.3%,91.0%,88.8%;抗SSA、抗SSB、抗Ro-52在干燥综合征中的检出率分别为72.1%,65.1%,67.4%;抗nRNP抗体在混合性结缔组织病中的检出率为77.8%;抗SCL-70抗体在系统性硬化症中的检出率为75.0%;抗AMA-M2抗体在原发性胆汁性肝硬化中的检出率为66.7%.[结论]不同AID患者血清中所含的自身抗体有差异,ANA和ENA联合检测对诊断AID具有重要意义.
OBJECTIVE To explore the diagnostic value of antinuclear antibodies(ANA)and anti-extractable nuclear antigen(ENA)polypeptide spectrum in the diagnosis of autoimmune disease(AID).METHODS ANA and anti-ENA levels were measured and analyzed in 381 patients with various autoimmune diseases and 100 healthy subjects.RESULTS ANA had the highest positive rate(95.5%)in patients with systemic lupus erythematosus(SLE),and the detection rates of anti-SSA,anti-SSB,anti-Sm,anti-dsDNA and anti-nucleosome were 84.8%,76.2%,36.3%,91.0%and 88.8%respectively.The detection rates of anti-SSA,anti-SSB and anti-Ro-52 in Sjogren syndrome were 72.1%,65.1%and 67.4%respectively.The detection rate of anti-nRNP antibody in mixed connective tissue disease was 77.8%.The detection rate of anti-SCL-70 antibody in systemic sclerosis was 75.0%.The detection rate of anti AMA-M2 antibody in primary biliary cirrhosis was 66.7%.CONCLUSION The autoantibodies in the serum of different AID patients were different.Combined detection of ANA and anti-ENA was of great significance for the diagnosis of AID.
作者
金春花
张洪江
姜春善
JIN Chunhua;ZHANG Hongjiang;JIANG Chunshan(Department of Laboratory,Affiliated Hospital of Yanbian University,Yanji 133000,Jilin,China)
出处
《延边大学医学学报》
CAS
2019年第4期292-294,共3页
Journal of Medical Science Yanbian University
关键词
自身免疫病
抗核抗体
抗可提取性核抗原
autoimmune diseases
antinuclear antibodies
anti-extractable nuclear antigen antibody
作者简介
金春花(1981-),女(朝鲜族),主管检验师,硕士研究生,研究方向为临床免疫学技术;通信作者:姜春善,Email:jcs0915@163.com.
