摘要
目的比较间接免疫荧光法（IIF）、酶联免疫吸附测定（ELISA）及放射免疫检测法（Farr）检测抗dsDNA抗体的敏感性及特异性，为开展临床联合检测寻找合适的方法。方法收集我院门诊和住院患者血标本共200份，其中包括门诊和住院及后期追踪经临床诊断的系统性红斑狼疮（SLE）120份标本、类风湿关节炎（RA）20份标本。进行性系统硬化症（PSS）20例。混合型结缔组织病（McTD）20例，干燥综合征（SS）20例，另健康体检者50例（全部经排查不具有结缔组织病）同时用以上三种方法检测抗dsDNA抗体。结果用IIF、ELISA及Farr检测抗dsDNA抗体，SLE患者抗dsDNA抗体阳性率分别为25％、32％和32％。RA患者抗dsDNA抗体阳性率均为0。PSS患者抗dsDNA抗体阳性率分别为0、0和5％。MCTD病抗dsDNA抗体阳性率分别为0、5％和10％。SS患者抗dsDNA抗体阳性率均为0。健康体检者抗dsDNA抗体阳性率均为0。结论用两种方法联合检测抗dsDNA抗体，尤其是IIF和ELISA检测，能提高单一一种方法对SLE患者抗dsDNA抗体检出率，有助于SLE的诊断及病情回顾。
Objective To compare the three Anti-dsDNA antibody detecting test （IIF, ELISA, Farr） with 200 serum samples to evaluate which one has higher sensitivity and specificity. Methods 200 serum samples including 120 serum samples of SLE, 20 serum samples of rheumatoid arthritis, 20 serum samples of MCTD, 20 serum samples of SS, 20 serum samples of PSS and 50 serum samples of healthy measured by IIF, Farr and ELISA. Results Detection the Anti-dsDNA antibody of the serum sample with the methods of IIF, ELISA and Farr. The positive percentage of Anti-dsDNA in SLE is 25%, 32% and 32%, while in RA is 0, 0 and 0; in laSS is 0, 0 and 5 % ; in SS is 0, 0 and 0 ; in healthy is O, 0 and O. Conclusion Detection the Anti-dsDNA antibody with two method in the same time, especially with IIF and ELISA, will heighten the positive rate than with single method and will be helpful for the diagnosis of SLE.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第1期74-75,共2页
Chinese Journal of Experimental and Clinical Virology
关键词
免疫荧光技术
间接
酶联免疫吸附测定
放射免疫检测
抗体
抗核
Fluorescent antibody technique
Enzyme-linked immunosorbent assay
Radioimmunoditection
Antibodies, antinuclear
作者简介
通信作者：佟大伟，Email：dawei_tong@163．com，电话：010-88068108
