摘要
探讨葛根芩连汤对腹泻型肠易激综合征(irritable bowel syndrome with diarrhea, IBS-D)大鼠肠道菌群的干预效果。将36只雄性SD大鼠按随机数字表法分为6组:正常组,模型组,利福昔明组(150 mg·kg^(-1)),葛根芩连汤高(8.125 g·kg^(-1))、中(4.062 5 g·kg^(-1))、低剂量组(2.031 3 g·kg^(-1)),每组6只。构建IBS-D大鼠模型,干预治疗8 d,观察大鼠一般状态和粪便性状评分、腹部收缩最小容量阈值;行HE染色观察结肠组织的病理学变化;利用16S rRNA测序观察肠道菌群变化。结果显示,与模型组比较,利福昔明组、葛根芩连汤高剂量组、葛根芩连汤中剂量组粪便性状评分均明显降低(P<0.01),腹部抬起最小容量阈值均显著升高(P<0.05)。HE染色显示葛根芩连汤干预后可以减轻肠道炎症。16S rRNA测序分析发现,IBS-D大鼠肠道菌群发生明显改变;葛根芩连汤干预后显著提高肠道菌群的丰富度指数与多样性指数,调节菌群物种数量,改善alpha多样性与beta多样性。肠道菌群物种组成及LEfSe分析显示,葛根芩连汤可以显著提高拟杆菌门Bacteroidota与厚壁菌门Firmicutes的比值,增加梭菌纲Clostridia、毛螺菌目Lachnospirales等益生菌丰度,减少拟杆菌目Bacteroidales、普雷沃菌科Prevotellaceae等条件致病菌丰度。PICRUSt2功能预测分析显示,葛根芩连汤主要与碳水化合物代谢、氨基酸代谢等多种代谢途径有关。综上所述,葛根芩连汤能够显著降低IBS-D大鼠内脏高敏性,减轻肠道炎症,改善腹泻等临床症状,其机制可能是通过调节肠道菌群组成结构并改善其代谢途径而发挥功效。
This study aims to explore the effect of Gegen Qinlian Decoction on gut microbiota of irritable bowel syndrome with diarrhea(IBS-D) rats. A total of 36 male SD rats were randomly classified into the control group, model group, rifaximin group(150 mg·kg^(-1)), and high-dose(8.125 g·kg^(-1)), medium-dose(4.062 5 g·kg^(-1)) and low-dose(2.031 3 g·kg^(-1)) Gegen Qinlian Decoction groups with the random number table method, 6 in each group. After modeling, rats were treated for 8 days. The general state, bristol stool chart(BSC) score, and the minimum volume threshold for abdominal withdrawal reflex were recorded. Pathological changes of colon tissues were observed based on hematoxylin and eosin(HE) staining, and gut microbiota was analyzed based on 16 S rRNA sequencing. Compared with the model group, rifaximin group and high-dose and medium-dose Gegen Qinlian Decoction groups showed low BSC score(P<0.01) and high minimum volume threshold for abdominal lifting(P<0.05). HE staining showed that Gegen Qinlian Decoction could relieve intestinal inflammation. 16 S rRNA sequencing suggested obvious variation of gut microbiota in IBS-D rats. Gegen Qinlian Decoction significantly raised the richness index and diversity index of gut microbiota, regulated the number of the flora, and improved alpha diversity and beta diversity. Species composition of gut microbiota and LEfSe analysis showed that Gegen Qinlian Decoction could significantly increase the ratio of Bacteroidota to Firmicutes, elevate the abundance of probiotics such as Clostridia and Lachnospirales, and reduce the abundance of conditional pathogens such as Bacteroidales, and Prevotellaceae. PICRUSt2 analysis indicated that Gegen Qinlian Decoction was mainly related to multiple metabolic pathways such as carbohydrate metabolism and amino acid metabolism. In summary, Gegen Qinlian Decoction can significantly reduce visceral hypersensitivity of IBS-D rats, alleviate intestinal inflammation, and relieve clinical symptoms such as diarrhea. The mechanism is the likelihood that it regulates the composition and structure of gut microbiota and improves its metabolic pathway as well.
作者
张儒奇
方志安
韩文庆
荆季群
刘艳
于斌
王荣
孙闵
ZHANG Ru-qi;FANG Zhi-an;HAN Wen-qing;JING Ji-qun;LIU Yan;YU Bin;WANG Rong;SUN Min(Jining Medical University,Jining 272067,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第24期6709-6719,共11页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81603509)
山东省自然科学基金项目(ZR2016HM65)
济宁医学院大学生创新训练计划项目(cx2021090)
济宁医学院中西医结合治疗脾胃病创新团队项目(jy201918)
作者简介
张儒奇,本科生,E-mail:zhangrq000@163.com;通信作者:孙闵,副教授,主要从事脾胃病临床研究,E-mail:sunmin0219@163.com
