To investigate the mechanism involved in the apoptosis of vascular smooth muscle cells induced by oxidized low density lipoprotein(OX LDL),cultured human vascular smooth muscle cells derived from umbilical artery were...To investigate the mechanism involved in the apoptosis of vascular smooth muscle cells induced by oxidized low density lipoprotein(OX LDL),cultured human vascular smooth muscle cells derived from umbilical artery were exposed to OX LDL.The apoptotic cells were determined by deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).The gene expressions of p53,p21,c fos and c jun were quantified by reverse transcription polymerase chain reaction(RT PCR).The contents of p53,p21,c fos and c jun were analyzed by immunochemical staining.The apoptotic vascular smooth muscle cells were found following treatment with OX LDL.The levels of p53,p21,c fos mRNA and protein were increased in vascular smooth muscle cells exposed to OX LDL.However,c jun expression remained constant.These results indicated that OX LDL could upregulate the expression of p53,p21 and c fos,inducing the apoptosis in vascular smooth muscle cells.展开更多
目的研究血清可替宁水平与冠心病的关系,探讨其在冠心病中的临床应用价值。方法按冠状动脉造影结果将149例研究对象分为冠心病组(97例)和非冠心病对照组(52例),进行病例对照研究,同位素稀释串联质谱法(isotope dilution tandem mass spe...目的研究血清可替宁水平与冠心病的关系,探讨其在冠心病中的临床应用价值。方法按冠状动脉造影结果将149例研究对象分为冠心病组(97例)和非冠心病对照组(52例),进行病例对照研究,同位素稀释串联质谱法(isotope dilution tandem mass spectrometry,ID-LC/MS/MS)测定血清可替宁浓度,分析可替宁与冠心病及其他危险因素的关系。结果 149例行冠状动脉造影患者血清可替宁浓度呈偏态和尖态分布(偏度2.90,峰度7.96),吸烟者的可替宁浓度为12.64(0.91～99.61)μg/L,显著高于非吸烟者[0.22(0.12～0.51)μg/L,P<0.001]。高可替宁水平组(>10.00μg/L)患冠心病的风险显著高于低可替宁水平组(<1.00μg/L)(OR=2.94,95%CI:1.11～7.78,P<0.05);且随着血清可替宁水平的升高,OR值增大呈量效关系(均为P<0.05)。多因素Logistic回归分析显示,糖尿病、血脂异常及可替宁为最显著的危险因素(均为P<0.05)。血清可替宁与吸烟(r=0.656,P<0.001)和白细胞计数(WBC,r=0.257,P=0.010)呈显著正相关,与HDL-C(r=-0.184,P=0.025)和HDL2-C(r=-0.217,P=0.008)呈显著负相关。结论血清可替宁水平能够有效地反映吸烟的暴露水平,其水平升高能够增加患冠心病的危险。展开更多
文摘To investigate the mechanism involved in the apoptosis of vascular smooth muscle cells induced by oxidized low density lipoprotein(OX LDL),cultured human vascular smooth muscle cells derived from umbilical artery were exposed to OX LDL.The apoptotic cells were determined by deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).The gene expressions of p53,p21,c fos and c jun were quantified by reverse transcription polymerase chain reaction(RT PCR).The contents of p53,p21,c fos and c jun were analyzed by immunochemical staining.The apoptotic vascular smooth muscle cells were found following treatment with OX LDL.The levels of p53,p21,c fos mRNA and protein were increased in vascular smooth muscle cells exposed to OX LDL.However,c jun expression remained constant.These results indicated that OX LDL could upregulate the expression of p53,p21 and c fos,inducing the apoptosis in vascular smooth muscle cells.
文摘目的研究血清可替宁水平与冠心病的关系,探讨其在冠心病中的临床应用价值。方法按冠状动脉造影结果将149例研究对象分为冠心病组(97例)和非冠心病对照组(52例),进行病例对照研究,同位素稀释串联质谱法(isotope dilution tandem mass spectrometry,ID-LC/MS/MS)测定血清可替宁浓度,分析可替宁与冠心病及其他危险因素的关系。结果 149例行冠状动脉造影患者血清可替宁浓度呈偏态和尖态分布(偏度2.90,峰度7.96),吸烟者的可替宁浓度为12.64(0.91～99.61)μg/L,显著高于非吸烟者[0.22(0.12～0.51)μg/L,P<0.001]。高可替宁水平组(>10.00μg/L)患冠心病的风险显著高于低可替宁水平组(<1.00μg/L)(OR=2.94,95%CI:1.11～7.78,P<0.05);且随着血清可替宁水平的升高,OR值增大呈量效关系(均为P<0.05)。多因素Logistic回归分析显示,糖尿病、血脂异常及可替宁为最显著的危险因素(均为P<0.05)。血清可替宁与吸烟(r=0.656,P<0.001)和白细胞计数(WBC,r=0.257,P=0.010)呈显著正相关,与HDL-C(r=-0.184,P=0.025)和HDL2-C(r=-0.217,P=0.008)呈显著负相关。结论血清可替宁水平能够有效地反映吸烟的暴露水平,其水平升高能够增加患冠心病的危险。
