利用GenBank中的数据,通过聚合酶链式反应(polymerase chain reaction,PCR)从人的cDNA文库中克隆得到功能基因TOX(thymocyte selection-associated high mobility group box),运用生物信息学分析其结构特性,并通过逆转录PCR(reverse tra...利用GenBank中的数据,通过聚合酶链式反应(polymerase chain reaction,PCR)从人的cDNA文库中克隆得到功能基因TOX(thymocyte selection-associated high mobility group box),运用生物信息学分析其结构特性,并通过逆转录PCR(reverse transcription PCR,RT-PCR)分析TOX在细胞系中的表达;利用荧光显微镜观察其亚细胞定位情况;使用细胞生长曲线实验和流式细胞术分析TOX对细胞增殖及细胞周期的影响.结果表明:TOX基因定位于人8号染色体q12.1上,全长4 131bp,编码526个氨基酸,该蛋白质分子质量约为57ku,含有9个外显子和8个内含子,其编码蛋白为高迁移率蛋白家族成员,能参与基因调控等生理过程;TOX基因在白血病细胞系Jurkat和Raji中高表达,并定位于细胞核中;对细胞生长曲线绘制及细胞周期分析表明,TOX能够使细胞生长速度加快,且S期细胞比例明显上升.说明TOX参与了细胞增殖调控,有可能研究开发为药物靶标或疾病标志物.展开更多
The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein ...The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein spots detected in the 2-DE maps from normal and autophagic MCF-7 cells respectively,among which 5 changed protein spots were identified by the Q-TOF MS/MS and database search.展开更多
文摘The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein spots detected in the 2-DE maps from normal and autophagic MCF-7 cells respectively,among which 5 changed protein spots were identified by the Q-TOF MS/MS and database search.
