摘要
目的:探讨汉族人种中维生素D受体(vitamin D receptor,VDR)基因多态性与牙周炎的关系。方法:选取90名侵袭性牙周炎患者、34名慢性牙周炎患者和91名健康对照者,抽取外周静脉血,提取基因组DNA,应用TaqⅠ限制性内切酶,采用聚合酶链反应-限制性片段长度多态性的方法检测VDR基因的多态性。结果:(1)Tt基因型在侵袭性牙周炎患者中的检出率高于健康对照者,但差异无统计学意义(16.7%vs7.7%,P=0.065),而在女性侵袭性牙周炎患者与女性健康对照者之间,Tt基因型频率的差异有统计学意义(20.8%vs4.2%,P<0.05);在男性侵袭性牙周炎患者与男性健康对照者间,Tt基因型的频率则几乎没有差异(10.8%vs11.6%,P>0.05);(2)在慢性牙周炎患者与健康对照者间,Tt基因型的频率差异无显著性(P>0.05)。结论:携带Tt型VDR基因可能是中国汉族女性患侵袭性牙周炎的危险因素。
Objective：To investigate the relationship between vitamin D receptor（VDR） gene polymorphisms and periodontitis.Methods： Ninety patients with aggressive periodontitis（AgP）,34 patients with chronic periodontitis and 91 healthy controls were recruited in this study.VDR gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） with TaqⅠ restriction endonuclease.Results： The detection frequency of Tt genotype was higher in the aggressive periodontitis patients than in the healthy controls（16.7% vs 7.7%,P〉0.05）.The difference between the female aggressive periodontitis patients and healthy controls（20.8% vs 4.2%,P〈0.05） was statistically significant,but no difference was found between the male patients and controls（10.8% vs 11.6%,P〉0.05）.There was a strong association between aggressive periodontitis and Tt genotype in females（AgP patients vs healthy controls,OR=6.02）.The detection frequency of TaqⅠ ER-α genotypes was not statistically different between the chronic periodontitis patients and healthy controls.Conclusion： In female Han Chinese population,the Tt VDR genotype may be associated with aggressive periodontitis.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2010年第1期37-40,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30271411)
北京大学人类疾病基因中心"985"项目(2001-8)
国家高技术研究发展计划专项经费(2002AA217091)资助~~
关键词
牙周炎
多态现象
遗传
受体
骨化三醇
Periodontitis
Polymorphism
genetic
Receptors.calcitriol
作者简介
Conesponding author＇s e-mail，kqhxmeng@mj．bjmu．edu.cn
