Background Aquaporins(AQPs)are integral membrane proteins belonging to the major intrinsic protein(MIP)family,playing a crucial role in water transport,cell elongation,and stress responses.However,their evolutionary d...Background Aquaporins(AQPs)are integral membrane proteins belonging to the major intrinsic protein(MIP)family,playing a crucial role in water transport,cell elongation,and stress responses.However,their evolutionary dynamics and functional roles in Gossypium species remain poorly characterized.Results In the present study,a total of 55,54,54,103,106,108,and 104 AQP genes were found in G.herbaceum,G.arboreum,G.raimondii,G.barbadense,G.tomentosum,G.mustelinum,and G.darwinii,respectively.Phylogenetic analysis classified them into five conserved subfamilies(PIP,TIP,NIP,SIP,and XIP),with 95 genes showing synteny across species and 17 displaying divergence,suggesting subgenome differentiation.Transcriptome analysis revealed that specific Gb AQP genes are involved in early salt stress responses and fiber development.Physiological assays demonstrated stronger salt tolerance in tetraploid cottons,particularly G.darwinii,compared with diploids.Co-expression network analysis linked AQPs to abiotic stress and fiber traits,and virus-induced gene silencing(VIGS)confirmed four AQP genes as critical for salt tolerance.Conclusion This study provides comprehensive insights into the evolution,expression,and functional roles of AQPs in cotton,identifying key candidate genes for improving salt tolerance and fiber quality in Gossypium species.展开更多
The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 ...The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 （3^4）] was used. Three factors and three levels were molecular weight of PEG： 8 000, 6 000, 4 000 mol ·g^-1; PEG concentration： 45%, 50%, 55%; treatment duration time： 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were： molecular weight 4 000, concentration 55%, treatment duration time： 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time→concentration→molecular weight.展开更多
Cotton is one of the most important economic crops in the world,and it p rovides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption de-mands on cotton fiber...Cotton is one of the most important economic crops in the world,and it p rovides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption de-mands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield and fiber quality are complex quantitative traits andare usually ne gatively associated that hampers the sim-ultaneous improvement of lint yield and fiber quality by traditional breeding.In an attempt to over"come the limitations of the conventional bree ding,molecular breeding based on marker-assisted selec-tion is a reality as marker technology has become cheaper and higher thro ughput.展开更多
Fluorescent in situ hybridization(FISH) is an important technique in plant genome research,because it provides integrated information about DNA,chromosomes and genomes.Genomic in situ hybridization(GISH) is a modifica...Fluorescent in situ hybridization(FISH) is an important technique in plant genome research,because it provides integrated information about DNA,chromosomes and genomes.Genomic in situ hybridization(GISH) is a modification of FISH that can be used to rapidly compare genome content,relatedness,organization and/or behavior.GISH results often provide insight into genome evolution and species relationships.The cotton genus Gossypium consists of about 50 species,including 5展开更多
Upland cotto n has the highest yield,and accounts for>95%of world cotton production.Deco-ding upland cotton genome s will undoubtedly provide the ultimate reference and resource for structur-al,functional,and evolu...Upland cotto n has the highest yield,and accounts for>95%of world cotton production.Deco-ding upland cotton genome s will undoubtedly provide the ultimate reference and resource for structur-al,functional,and evolutio nary studies of the species.Here,we employed GeneTrek and BAC tag-ging information approaches to predict the general composition and structure of the allotetraploid cot-ton genome.Further,based on our enhanced genetic map between Gossy pium barba dense and G.hir-sutum,we carried out the integration of 26 genetic and physical maps from G.hirsutum by chromo-some-specific BACs and FISH technology and put a foundation for BAC-byBAC sequencing from ho-mologous chr.12 and chr.26.142 BAC sequences from G.展开更多
QTL analyses were performed in tetraploid cotton.An interspecific F2 population consisting of 69 plants,which was developed from the cross between Gossypium hirsutum L.,cv.Handan 208(characterized as high fiber yield)...QTL analyses were performed in tetraploid cotton.An interspecific F2 population consisting of 69 plants,which was developed from the cross between Gossypium hirsutum L.,cv.Handan 208(characterized as high fiber yield)and G.barbadense L.,cv.Pima 90(characterized as excellent fiber quality),was genotyped with SSR,RAPD,SRAP,and REMAP markers.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32460522,32372128,and 32201713)。
文摘Background Aquaporins(AQPs)are integral membrane proteins belonging to the major intrinsic protein(MIP)family,playing a crucial role in water transport,cell elongation,and stress responses.However,their evolutionary dynamics and functional roles in Gossypium species remain poorly characterized.Results In the present study,a total of 55,54,54,103,106,108,and 104 AQP genes were found in G.herbaceum,G.arboreum,G.raimondii,G.barbadense,G.tomentosum,G.mustelinum,and G.darwinii,respectively.Phylogenetic analysis classified them into five conserved subfamilies(PIP,TIP,NIP,SIP,and XIP),with 95 genes showing synteny across species and 17 displaying divergence,suggesting subgenome differentiation.Transcriptome analysis revealed that specific Gb AQP genes are involved in early salt stress responses and fiber development.Physiological assays demonstrated stronger salt tolerance in tetraploid cottons,particularly G.darwinii,compared with diploids.Co-expression network analysis linked AQPs to abiotic stress and fiber traits,and virus-induced gene silencing(VIGS)confirmed four AQP genes as critical for salt tolerance.Conclusion This study provides comprehensive insights into the evolution,expression,and functional roles of AQPs in cotton,identifying key candidate genes for improving salt tolerance and fiber quality in Gossypium species.
文摘The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 （3^4）] was used. Three factors and three levels were molecular weight of PEG： 8 000, 6 000, 4 000 mol ·g^-1; PEG concentration： 45%, 50%, 55%; treatment duration time： 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were： molecular weight 4 000, concentration 55%, treatment duration time： 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time→concentration→molecular weight.
文摘Cotton is one of the most important economic crops in the world,and it p rovides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption de-mands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield and fiber quality are complex quantitative traits andare usually ne gatively associated that hampers the sim-ultaneous improvement of lint yield and fiber quality by traditional breeding.In an attempt to over"come the limitations of the conventional bree ding,molecular breeding based on marker-assisted selec-tion is a reality as marker technology has become cheaper and higher thro ughput.
文摘Fluorescent in situ hybridization(FISH) is an important technique in plant genome research,because it provides integrated information about DNA,chromosomes and genomes.Genomic in situ hybridization(GISH) is a modification of FISH that can be used to rapidly compare genome content,relatedness,organization and/or behavior.GISH results often provide insight into genome evolution and species relationships.The cotton genus Gossypium consists of about 50 species,including 5
文摘Upland cotto n has the highest yield,and accounts for>95%of world cotton production.Deco-ding upland cotton genome s will undoubtedly provide the ultimate reference and resource for structur-al,functional,and evolutio nary studies of the species.Here,we employed GeneTrek and BAC tag-ging information approaches to predict the general composition and structure of the allotetraploid cot-ton genome.Further,based on our enhanced genetic map between Gossy pium barba dense and G.hir-sutum,we carried out the integration of 26 genetic and physical maps from G.hirsutum by chromo-some-specific BACs and FISH technology and put a foundation for BAC-byBAC sequencing from ho-mologous chr.12 and chr.26.142 BAC sequences from G.
文摘QTL analyses were performed in tetraploid cotton.An interspecific F2 population consisting of 69 plants,which was developed from the cross between Gossypium hirsutum L.,cv.Handan 208(characterized as high fiber yield)and G.barbadense L.,cv.Pima 90(characterized as excellent fiber quality),was genotyped with SSR,RAPD,SRAP,and REMAP markers.
