摘要
The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 （3^4）] was used. Three factors and three levels were molecular weight of PEG： 8 000, 6 000, 4 000 mol ·g^-1; PEG concentration： 45%, 50%, 55%; treatment duration time： 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were： molecular weight 4 000, concentration 55%, treatment duration time： 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time→concentration→molecular weight.
The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L9 （3^4）] was used. Three factors and three levels were molecular weight of PEG： 8 000, 6 000, 4 000 mol ·g^-1; PEG concentration： 45%, 50%, 55%; treatment duration time： 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were： molecular weight 4 000, concentration 55%, treatment duration time： 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time→concentration→molecular weight.
基金
National 863 High-technology (863-819-01-04)
作者简介
LI Yajuan （1961-）, female, master, associate professor, engaged in the reseach of animal genetics and breeding.
